Kev kuaj mob cov khoom siv rau zis microalbumin

(Fluorescence imtimochromatogromographic ensay)

Rau hauv vitro kuaj xyuas siv nkaus xwb

Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.

Siv

Kev kuaj mob cov khoom siv rau zis microalbuminic (fluorescatogroma) Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb.

Lub ntsiab

Microalbumin yog ib qho protein ib txwm muaj nyob hauv cov ntshav thiab yog qhov tsis tshua muaj tsawg hauv cov zis thaum metabolized ib txwm muaj. Yog tias muaj cov nqi laij tawm hauv cov zis uas muaj ntau tshaj 20 micron, yog tias tuaj yeem tsim cov tshuaj tiv thaiv kab mob ntshav qab zib me, yog tias tuaj yeem siv cov tshuaj tiv thaiv kab mob ntshav qab zib me, yog tias tuaj yeem siv cov tshuaj tiv thaiv kab mob ntshav qab zib, yog tias tuaj yeem siv cov tshuaj tiv thaiv kab mob ntshav qab zib, yog tias tuaj yeem nkag rau hauv cov ntshav qab zib nephroary. Tus mob tuaj yeem kuaj pom tseeb raug yog raug tshuaj xyuas los ntawm cov etrary microalbumin, ua ke nrog qhov tshwm sim, cov tsos mob thiab keeb kwm kev kho mob. Ntxov nrhiav kom tau tso zis ntawm microalbumin yog ib qho tseem ceeb heev kom tiv thaiv thiab ncua kev txhim kho cov ntshav qab zib Mephotropathy.

Lub hauv paus ntsiab lus ntawm cov txheej txheem

Daim nyias nyias ntawm cov cuab yeej ntsuas yog coated nrog Alb antigen ntawm thaj chaw xeem thiab tshis Anti Lij igg antibodody ntawm thaj chaw tswj. Marker Pad yog coated los ntawm fluorescence los tiv thaiv alb quav thiab luav IGG ua ntej. Thaum kuaj cov qauv, alb hauv cov qauv ua ke nrog fluorescence anti alb antibody, thiab kev sib xyaw ua paug. Nyob rau hauv qhov kev txiav txim ntawm immunochrataphyog teb, cov complex ntws ntawm albbres, thiab kev ua kom zoo nkauj ntawm cov qauv tshuaj ntsuam, yog qhov pom tau fluorescent ntawm fluorescence los xij.

Reagents thiab khoom siv

25T Pob Cheebtsam:

Kev Xeem Daim Npav Mus Rau Qhov Nyiaj Folded nrog Desiccant 25T

Pob ntxig 1

Cov ntaub ntawv yuav tsum tau tab sis tsis muab

Qauv sau ntim rau, timer

Qauv Sau thiab Cia

1. Cov qauv kuaj tuaj yeem yog zis.
2. Cov tshuaj ntsuab tshiab tuaj yeem sau hauv cov thawv ntim khoom huv. Nws raug nquahu kom sim cov zis tso zis tam sim ntawd tom qab sau. Yog hais tias cov zis kuaj tsis tuaj yeem kuaj tam sim ntawd, thov khaws lawv ntawm 2-8℃, tab sis nws pom zoo kom tsis txhob uae lawv rau ntau tshaj 12 teev. Tsis txhob co lub thawv. Yog tias muaj cov sediment nyob hauv qab ntawm lub ntim, noj supernatant rau kev sim.
3. Txhua yam piv txwv tsis txhob khov-thaw mus.
4. Thaws cov qauv rau chav sov ua ntej siv.