Kev kuaj mob cov khoom siv rauCalprotectin(Fluorescence imtimochromatogromographic ensay)
Rau hauv vitro kuaj xyuas siv nkaus xwb
Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.

Siv
Kev kuaj mob pob khoom siv rau Calprotectin (Cal) yog tsim nyog rau kev txiav txim siab ntawm tib neeg cov tshuaj ntsuam xyuas cov nqi rau kev ua kom tau zoo. Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb.

Lub ntsiab
Cal yog Heterodimer, uas yog muaj ntawm MRP 8 thiab mrp 14^[1]Cov. Nws muaj nyob rau hauv neutrophils cytoplasm thiab qhia tawm ntawm mononuclear cell wee. Cal yog mob theem cov protein, nws muaj ib qho kev ua tau zoo ncua ntev li ib lub lis piam hauv tib neeg cov tshuaj lom zem^[2-3]Cov. Cov khoom siv yog qhov yooj yim, kuaj pom kev ntsuam xyuas uas pom tias muaj cal rau tib neeg cov tshuaj qab cia, nws muaj cov siab nrhiav tau thiab muaj feem ntau. Qhov kev xeem raws li cov tshuaj tiv thaiv sandwich thiab fluorescatogratogromogratogromographic thev naus laus zis, uas tuaj yeem muab tau li ntawm 15 feeb.

Lub hauv paus ntsiab lus ntawm cov txheej txheem
Cov Sawb tau tiv thaiv cov tshuaj tiv thaiv tiv thaiv ntawm thaj av sim, uas tau ua kom nrawm rau membrane txoj kev ua kom nruj. Lable Pad yog coated los ntawm fluorescence sau cov tshuaj tiv thaiv tiv thaiv ua ntej. Thaum kuaj cov qauv zoo, tus cal nyob rau hauv cov qauv tuaj yeem sib xyaw nrog fluorescence sau cov tshuaj tiv thaiv tiv thaiv, thiab tshuaj tiv thaiv kab mob tiv thaiv kab mob, thiab tshuaj tiv thaiv kab mob tiv thaiv. Raws li cov sib tov yog tso cai rau kev tsiv teb tsaws chaw ntawm qhov kev sim ua ke yog ntes los ntawm Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Anti-Cal Ord Cov fluorescence kev siv hluav taws xob yog qhov sib thooj nrog cov ntsiab lus. Lub cal hauv cov qauv tuaj yeem pom tau los ntawm fluorescence txhaj tshuaj tiv thaiv kev txhaj tshuaj tiv thaiv kab ntsig.

Reagents thiab khoom siv

25T Pob Cheebtsam:
Kev Xeem Daim Npav Mus Rau Qhov Nyiaj Folded nrog Desiccant 25T
Qauv Diluents 25T
Pob ntxig 1

Cov ntaub ntawv yuav tsum tau tab sis tsis muab
Qauv sau ntim rau, timer

Qauv Sau thiab Cia
1. Siv cov thawv ntim khoom huv pov tseg kom sau cov qauv dej tshiab, thiab kuaj tam sim ntawd. Yog tias tsis raug kuaj tam sim ntawd, thov khaws cia ntawm 2-8 ° C rau 3 hnub lossis qis dua-15 ° C rau 6 lub hlis.
2.Thaum cov qauv sampling, ntxig rau hauv cov qauv hauv 3 zaug, nqa cov qauv uas muaj ntau zaus, tom qab ntawd, ntsia hlau nruj thiab siv cov qauv sib tw khaws Txog 50mg faeces qauv, thiab muab tso rau hauv ib faeces qauv tube uas muaj cov qauv siv, thiab ntsia hlau nruj.
3. Siv cov pipette uas siv tau ua piv txwv los ntawm tus neeg mob raws plab tus neeg mob, tom qab ntawd ntxiv 3μl) rau cov quav tsis sib xws ntawm lub raj thiab co kom zoo.

Sau ntawv:
1.Navoid khov-thaw mus.
2.Qhov cov qauv rau chav sov ua ntej siv.

Txheej txheem assay

Thov nyeem cov lus qhia ua haujlwm thiab cov ntim ntim ua ntej xeem.
1.Txhua txhua yam tshuaj tiv thaiv thiab cov hnoos qeev mus rau hnub sov.
2.Op Lub portable antalable artalyzer (Wiz-A101), nkag mus rau tus lej nyiaj nkag mus raws cov khoom lag luam, thiab nkag mus nrhiav qhov kev tshawb pom.
3.Can Cov kws tu hniav rau cov kws kho hniav kom paub meej tias cov khoom xeem.
4.Txhua daim npav xeem los ntawm lub hnab nyiaj.
5.insert daim npav xeem rau hauv daim npav qhov, scan tus QR code, thiab txiav txim siab cov khoom xeem.
6.Qhov lub hau los ntawm tus qauv raj thiab muab pov tseg thawj ob drops diluted qauv ntsug thiab maj mam ua cov qauv zoo ntawm daim npav nrog muab cov khoom xa tawm.
7.Click "tus qauv xeem", tom qab 15 feeb, qhov ntsuas yuav pom tau cov txiaj ntsig los ntawm cov khoom siv tshuaj ntsuam, thiab sau cov ntaub ntawv xeem.
8.Reer rau cov lus qhia ntawm lub portable ageler (wiz-a101).

Cia siab tias muaj txiaj ntsig
Cal <60μg / g
Nws raug nquahu tias txhua lub chaw kuaj xyuas tsim nws tus kheej li qub uas nws tus neeg mob ib txwm muaj.

Kuaj cov qhabnias thiab kev txhais lus
1.Qhov cal hauv cov qauv yog ntau ntxiv uas 60μg / g, thiab yuav tsum txiav txim tawm cov kev hloov pauv ntawm lub xeev lub siab. Cov txiaj ntsig yog tseeb txawv txav thiab yuav tsum tau kuaj xyuas nrog cov tsos mob hauv cov tsos mob.
2.Qhov cov txiaj ntsig ntawm cov qauv no tsuas yog siv rau cov kev siv tau tsim nyob rau hauv hom no, thiab tsis muaj qhov sib piv ncaj qha nrog lwm txoj hauv kev.
3. Ua rau cov yam ntxwv tsis muaj peev xwm ua tau rau qhov tsis raug rau cov txiaj ntsig tau pom, suav nrog cov laj thawj, kev ua haujlwm thiab lwm yam qauv.

Cia thiab Ruaj Khwv
1.Qhov khoom siv yog 18 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis tau ntawm 2-30 ° C. Tsis khov. Tsis txhob siv dhau hnub tas sij hawm.
2.Tsis txhob qhib lub hnab ntim khoom mus txog thaum koj tau npaj los ua ib qho kev kuaj mob (kub 2-35 ℃, av noo 40-90%) tsis pub dhau 60 mins sai li sai tau li sai tau.
3.Sample Diluent yog siv tam sim ntawd tom qab qhib.

Ceeb Toom thiab Ceev Faj
Cov .Qhov khoom siv yuav tsum tau muab khi thiab tiv thaiv tiv thaiv noo noo.
.Txhua cov khoom zoo yuav tsum raug muab los ntawm lwm cov txheej txheem.
.All cov txheej txheem yuav raug kho kom zoo li muaj kuab paug.
.Do tsis siv reagent reagent.
.Do tsis tau sib pauv reagents ntawm cov khoom siv nrog sib txawv ntau tsis tau ..
.Do tsis siv daim npav xeem thiab cov khoom siv pov tseg.
.Misoperation, ntau dhau los yog me me qauv tuaj yeem ua rau muaj kev cuam tshuam.

Kev txwv
.As nrog ib qho zoo li cov tshuaj tua kab mob nas, qhov ua tau tshwm sim los ntawm tib neeg kev tawm tsam nas tua kab mob (HAMA) hauv qhov quav. Cov quav dev los ntawm cov neeg mob uas tau txais kev npaj ntawm monoclonhal tshuaj tiv thaiv rau kev kuaj mob lossis kev kho mob tuaj yeem muaj Hama. Cov hnoos qeev no yuav ua rau cov txiaj ntsig zoo lossis tsis tseeb.
.Qhov kev xeem ua tsuas yog rau kev kuaj mob, yuav tsum tsis txhob txiav txim siab ua ke nrog nws cov tsos mob hauv kev kho mob, kev kuaj mob, Epidemiology thiab lwm yam ntaub ntawv Cov.
.This reagent tsuas yog siv rau kev kuaj quav. Tej zaum nws yuav tsis tau txais qhov tshwm sim raug thaum siv rau lwm qhov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.

Cov yam ntxwv ua tau zoo

Ntawv Sawv cev
1.Li, g. & Y.l.li.Qhov kev sib raug zoo ntawm kab mob calcium thiab vindoty ntawm cov tswv yim siv, 2007,23 (15)
2.han, W., Xu
3.Tej, ZH, Gu, thiab, li Al.The kawm txog kev sib raug zoo ntawm kev sib raug zoo thiab mob hnyuv [j]. Science technology thiab engineering, 2010-03,10 (8)

Qhov tseem ceeb rau cov cim siv:

	Hauv cov vitro kuaj kho mob kho mob
	Tus tsim
	Khw ntawm 2-30 ℃
	Hnub tag sijhawm
	Tsis txhob rov siv dua
	Ceev faj
	Tham cov lus qhia kev siv

Xiamen Wiz Biotech CO., Ltd
Chaw Nyob: 3-4 pem teb, no.16 lub tsev, Rio-Medhoo Rhiav, 2030 Wengjiao West Txoj Kev, Lub Tsev Kawm Ntawv Hengeviao, 361026, Xiamen, Suav
Tel: + 86-592-6808278
Fax: + 86-592-6808279