Diagnostic Kit rauCalprotectin(Fluorescence Immunochromatographic Assay)
Rau kev kuaj mob hauv vitro nkaus xwb
Thov nyeem cov pob no kom zoo ua ntej siv thiab ua raws li cov lus qhia nruj me ntsis. Kev ntseeg siab ntawm cov txiaj ntsig kev ntsuas tsis tuaj yeem lav tau yog tias muaj kev sib txawv ntawm cov lus qhia hauv pob no.

YUAV TSUM SIV
Diagnostic Kit rauCalprotectin(Cal) yog qhov tsim nyog rau kev txiav txim siab ntau ntawm tib neeg faeces Cal los ntawm fluorescence immunochromatographic assay, uas muaj qhov tseem ceeb accessory diagnostic tus nqi rau inflammatory plob tsis so tswj kab mob.Txhua tus qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev sim no yog npaj rau kev siv kws kho mob nkaus xwb.

TSEEM CEEB
Cal yog ib qho heterodimer, uas yog tsim los ntawm MRP 8 thiab MRP 14^[1]. Nws muaj nyob rau hauv neutrophils cytoplasm thiab nthuav tawm ntawm mononuclear cell membranes. Cal yog mob theem proteins, nws muaj ib tug ruaj khov theem txog ib lub lim tiam nyob rau hauv tib neeg faeces, nws txiav txim siab los ua ib tug inflammatory plob tsis so tswj kab mob.^[2-3]. Cov khoom siv yog qhov yooj yim, pom qhov ntsuas qhov ntsuas uas pom Cal nyob rau hauv tib neeg faeces, nws muaj kev tshawb pom siab thiab muaj zog tshwj xeeb. Qhov kev ntsuam xyuas raws li qhov tshwj xeeb ntawm ob chav tshuaj tiv thaiv kab mob Sandwich tshuaj tiv thaiv txoj cai thiab fluorescence immunochromatographic tshuaj ntsuam xyuas technics, uas tuaj yeem muab cov txiaj ntsig hauv 15 feeb.

TSEV KAWM NTAWV NO
Lub sawb muaj cov tshuaj tiv thaiv-Cal txheej antibody ntawm cheeb tsam kuaj, uas yog fastened rau daim nyias nyias chromatography ua ntej. Lable pad yog coated los ntawm fluorescence sau los tiv thaiv Cal antibody ua ntej. Thaum kuaj cov qauv zoo, Cal hauv cov qauv tuaj yeem sib xyaw nrog cov tshuaj fluorescence sau los tiv thaiv Cal antibody, thiab tsim cov tshuaj tiv thaiv kab mob. Raws li qhov sib xyaw tau tso cai rau kev tsiv teb tsaws raws txoj kab kev sim, Cal conjugate complex yog ntes los ntawm kev tiv thaiv Cal txheej antibody ntawm daim nyias nyias thiab cov ntaub ntawv complex. Qhov kev siv fluorescence zoo sib xws nrog Cal ntsiab lus. Cov Cal hauv cov qauv tuaj yeem kuaj pom los ntawm fluorescence immunoassay analyzer.

REAGENTS THIAB KHOOM PLIG

25T pob khoom:
Daim npav xeem ntawv ib tus zuj zus tuaj nrog lub desiccant 25T
Qauv diluents 25T
Pob ntawv ntxig 1

Cov khoom yuav tsum tau tab sis tsis muab
Sample ntim thawv, timer

SAMPLE COLLECTION THIAB STORAGE
1.Siv lub thawv huv huv los khaws cov qauv faeces tshiab, thiab sim tam sim ntawd. Yog tias tsis tuaj yeem kuaj tam sim ntawd, thov khaws cia ntawm 2-8 ° C rau 3 hnub lossis qis dua -15 ° C rau 6 lub hlis.
2. Tshem tawm cov qauv coj mus kuaj, muab tso rau hauv cov qauv faeces, rov ua qhov kev txiav txim 3 zaug, coj qhov sib txawv ntawm cov faeces coj mus kuaj txhua lub sijhawm, tom qab ntawd muab cov sampling stick rov qab, ntsia hlau nruj thiab co zoo, Los yog siv cov sampling stick khaws. kwv yees li 50mg faeces qauv, thiab muab tso rau hauv lub faeces qauv raj uas muaj cov qauv dilution, thiab ntsia hlau nruj.
3. Siv cov khoom siv pov tseg pov tseg coj cov qauv faeces los ntawm tus neeg mob raws plab, tom qab ntawd ntxiv 3 tee (kwv yees li 100µL) rau hauv lub raj fecal sampling thiab co zoo.

Nco tseg:
1.Tsis txhob khov-thaw mus.
2.Thaw cov qauv mus rau chav sov ua ntej siv.

TSEEM CEEB

Thov nyeem phau ntawv ntsuas kev ua haujlwm thiab pob ntim ua ntej kuaj.
1. Tso tag nrho cov reagents thiab cov qauv mus rau chav tsev kub.
2.Qhib lub Portable Immune Analyzer (WIZ-A101), nkag mus rau tus account password tus ID nkag mus raws li txoj kev ua haujlwm ntawm lub cuab yeej, thiab nkag mus rau qhov kev kuaj pom.
3.Scan lub dentification code kom paub meej tias cov khoom kuaj.
4. Tshem tawm daim ntawv xeem los ntawm lub hnab ntawv ci.
5.Insert daim npav xeem rau hauv daim npav, luam theej duab QR code, thiab txiav txim siab cov khoom xeem.
6. Tshem lub hau los ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 3 tee (li 100uL) tsis muaj npuas diluted qauv verticaly thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette.
7.Nias lub pob "standard test", tom qab 15 feeb, lub ntsuas yuav cia li ntes daim npav xeem, nws tuaj yeem nyeem cov txiaj ntsig ntawm lub vijtsam zaub ntawm lub ntsuas, thiab sau / luam cov txiaj ntsig kev xeem.
8.Saib cov lus qhia ntawm Portable Immune Analyzer (WIZ-A101).

TXOJ CAI ZOO
Cal <60 μg / g
Nws raug pom zoo tias txhua lub chaw kuaj mob tsim nws tus kheej ib txwm sawv cev rau nws cov neeg mob.

TEST RESULTS THIAB INTERPRETATION
1.Cov Cal nyob rau hauv cov qauv yog ntau tshaj 60μg / g, thiab yuav tsum tau txiav txim qhov kev hloov ntawm lub physiological lub xeev. Cov txiaj ntsig yog qhov txawv txav thiab yuav tsum tau kuaj xyuas cov tsos mob.
2.Cov txiaj ntsig ntawm txoj kev no tsuas yog siv tau rau cov kev siv ntau yam tsim los ntawm cov qauv no, thiab tsis muaj kev sib piv ncaj qha nrog lwm txoj kev.
3.Lwm yam tseem ceeb kuj tuaj yeem ua rau muaj qhov yuam kev hauv kev tshawb nrhiav, suav nrog cov laj thawj kev, kev ua haujlwm tsis raug thiab lwm yam qauv.

KHOOM THIAB STABILITY
1.Cov khoom siv yog 18 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis siv ntawm 2-30 ° C. TSIS PUB DAWB. Tsis txhob siv dhau hnub tas sij hawm.
2.Tsis txhob qhib lub hnab kaw kom txog thaum koj npaj txhij los ua qhov kev sim, thiab qhov kev sim ib zaug tau pom zoo kom siv nyob rau hauv ib puag ncig uas xav tau (kub 2-35 ℃, av noo 40-90%) hauv 60 mins sai raws li ua tau.
3.Sample diluent yog siv tam sim tom qab qhib.

CEEB TOOM THIAB KEV TIV THAIV
.Cov khoom siv yuav tsum tau muab kaw thiab tiv thaiv kom tsis txhob ya raws.
.Txhua cov qauv zoo yuav tsum raug lees paub los ntawm lwm cov txheej txheem.
.Txhua tus qauv yuav tsum raug kho raws li qhov muaj kuab paug.
.Tsis txhob siv cov reagent tas sij hawm.
.Tsis txhob hloov cov tshuaj reagents ntawm cov khoom siv sib txawv No..
.Tsis txhob rov siv cov ntawv xeem thiab cov khoom siv pov tseg.
.Misoperation, ntau dhau los yog me me qauv tuaj yeem ua rau muaj qhov sib txawv.

TXOJ CAI
Raws li nrog txhua qhov kev ntsuam xyuas uas siv cov tshuaj tiv thaiv nas, qhov ua tau tshwm sim rau kev cuam tshuam los ntawm tib neeg cov tshuaj tiv thaiv nas (HAMA) hauv cov qauv. Cov qauv ntawm cov neeg mob uas tau txais kev npaj ntawm monoclonal antibodies rau kev kuaj mob lossis kev kho mob yuav muaj HAMA. Cov qauv zoo li no tuaj yeem ua rau cov txiaj ntsig tsis zoo lossis tsis muaj tseeb.
.Qhov kev ntsuam xyuas no tsuas yog siv rau kev soj ntsuam xwb, yuav tsum tsis txhob ua lub hauv paus rau kev kuaj mob thiab kev kho mob nkaus xwb, cov neeg mob kev tswj xyuas kev kho mob yuav tsum muaj kev txiav txim siab ua ke nrog nws cov tsos mob, keeb kwm kev kho mob, lwm yam kev kuaj mob, kev kho mob teb, kab mob sib kis thiab lwm yam ntaub ntawv. .
.Qhov no reagent tsuas yog siv rau kev kuaj quav. Tej zaum nws yuav tsis tau txais txiaj ntsig zoo thaum siv rau lwm cov qauv xws li qaub ncaug thiab zis thiab lwm yam.

TXOJ CAI TSHIAB

REFERENCES
1.Li,G.&Y.L.Li.Kev sib raug zoo ntawm calcium thiab kev kho mob kab mob[J].The Journal of Practical Medicine,2007,23(15)
2.Han, W., Xu, JM, et al. Kev tshawb fawb soj ntsuam ntawm calamine thiab lactoferrin nyob rau hauv cov neeg mob ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang, ZH, Guo, HB, thiab al.Kev kawm txog kev sib raug zoo ntawm fecal calamine thiab inflammatory plob tsis so tswj kab mob [J]. Science Technology thiab Engineering, 2010-03,10(8)

Ntsiab rau cov cim siv:

	Hauv Vitro Diagnostic Medical Device
	Chaw tsim tshuaj paus
	Khaws ntawm 2-30 ℃
	Hnub Kawg
	Tsis txhob rov siv dua
	CEEB TOOM
	Tshawb xyuas cov lus qhia rau kev siv

Xiamen Wiz Biotech Co., Ltd
Chaw Nyob: 3-4 Pem Teb, NO.16 Lub Tsev, Bio-kev kho mob Rhiav, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Suav teb
Xov tooj: + 86-592-6808278
Tus Fax: + 86-592-6808279