Kev kuaj mob cov khoom siv rauhypersensitive C-reactive protein

(fluorescence imtimochromatogromographic ensay)

Rau hauv vitro kuaj xyuas siv nkaus xwb

Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.

Siv

Kev kuaj mob cov khoom siv rau hypersensitive C-reactive protein (fluorescatogromboard assay) yog fluorescatographic assay) yog cov ntshav siab (ntshav / ntshav / tag nrho cov ntshav. Nws yog qhov ntsuas tsis tau qhia tshwj xeeb. Txhua tus qauv zoo yuav tsum tau lees paub los ntawm lwm cov txheej txheem. Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb.

Lub ntsiab

C-reactive protein yog ib feem protein protein tsim los ntawm cov qog ua haujlwm ntawm daim siab thiab epithelial hlwb. Nws muaj nyob rau hauv tib neeg dej hiav txwv, cov kua dej Cleiabros, pelural thiab plab zom mov, thiab lwm yam ntawm cov tshuaj tiv thaiv kab mob tsis sib xws. 6-8H tom qab tshwm sim ntawm tus kab mob kis tau tshwm sim, CRP pib nce ntxiv, 24-48H mus txog lub ncov, thiab lub ncov siab yuav ncav tau ntau pua lub sijhawm ntawm ib txwm muaj. Tom qab tshem tawm ntawm tus kab mob, CRP poob loj hlob thiab rov qab mus rau ib txwm nyob hauv ib lub lis piam. Txawm li cas los xij, CRP tsis nce ntau qhov kis tau tus mob, uas muab cov kab mob rau kev txheeb xyuas cov kab mob thaum ntxov

Lub hauv paus ntsiab lus ntawm cov txheej txheem

Daim nyias nyias ntawm cov cuab yeej ntsuas yog coated nrog anti cr Tshuaj tiv thaiv nyob rau thaj tsam xeem thiab tshis los tiv thaiv thaj tsam ntawm cheeb tsam tswj. Lable Pad yog coated los ntawm fluorescence sau los anti cr Tshuaj tiv thaiv thiab cov luav IGG ua ntej. Thaum kuaj cov qauv zoo, CRP antigen hauv cov qauv ua ke nrog fluorescence los anti cr Tshuaj tiv thaiv kab mob, thiab tsim kev tiv thaiv kab mob tiv thaiv kab mob. Nyob rau hauv qhov kev txiav txim ntawm immunochromium, cov complex ntws ntawm cov ntawv nqus, thaum ua ke nrog anti crp txheej tshuaj tiv thaiv antibody, cov ntaub ntawv tshiab. CRP qib yog qhov sib thooj nrog cov teeb liab fluorescence, thiab cov concentration ntawm CRP hauv cov qauv tuaj yeem tuaj yeem kuaj pom los ntawm fluorescence imunoassay assay.

Reagents thiab khoom siv

25T Pob Cheebtsam:

Kev Xeem Daim Npav Mus Rau Qhov Nyiaj Folded nrog Desiccant 25T

Qauv Diluents 25T

Pob ntxig 1

Cov ntaub ntawv yuav tsum tau tab sis tsis muab

Qauv sau ntim rau, timer

Qauv Sau thiab Cia

1. Cov qauv kuaj tuaj yeem yog ntshav, heparin anticoagulant ntshav lossis edta anticoagulant ntshav.
2. Raws li cov qauv txheej txheem sau cov qauv. Ntshav dej lossis cov qauv ntshav lossis cov qauv ntshav lossis cov qauv ua tub rog tuaj yeem khaws cia hauv tub yees ntawm 2-8 ℃ rau 7days thiab cryoprimerve hauv qab -15 ° C rau 6 lub hlis. Tag nrho cov ntshav ntshav tuaj yeem khaws cia nyob hauv tub yees ntawm 2-8 ℃ rau 3 hnub
3. Txhua yam piv txwv tsis txhob khov-thaw mus.