Cov Khoom Siv Kuaj Mob rauC-reactive protein uas rhiab heev

(Kev ntsuam xyuas fluorescence immunochromatographic)

Rau kev siv kuaj mob hauv vitro xwb

Thov nyeem daim ntawv qhia no kom zoo ua ntej siv thiab ua raws li cov lus qhia. Kev ntseeg tau ntawm cov txiaj ntsig ntawm kev sim tsis tuaj yeem lav yog tias muaj kev hloov pauv ntawm cov lus qhia hauv daim ntawv qhia no.

YUAV TSUM SIV LI CAS

Kev Tshuaj Xyuas Khoom Siv rau hypersensitive C-reactive protein (fluorescence immunochromatographic assay) yog ib qho kev tshuaj ntsuam fluorescence immunochromatographic rau qhov ntau ntawm C-reactive protein (CRP) hauv tib neeg cov ntshav / plasma / Tag nrho cov ntshav. Nws yog ib qho cim qhia tsis yog qhov tshwj xeeb ntawm kev o. Tag nrho cov qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev xeem no yog rau kev siv kho mob xwb.

LUS QHIA

C-reactive protein yog ib qho protein theem mob uas tsim los ntawm kev txhawb nqa lymphokine ntawm daim siab thiab cov hlwb epithelial. Nws muaj nyob rau hauv tib neeg cov ntshav, cov kua dej cerebrospinal, pleural thiab plab kua, thiab lwm yam, thiab yog ib feem ntawm cov txheej txheem tiv thaiv kab mob tsis tshwj xeeb. 6-8 teev tom qab muaj kab mob bacterial, CRP pib nce, 24-48 teev mus txog qhov siab tshaj plaws, thiab qhov siab tshaj plaws tuaj yeem ncav cuag ntau pua npaug ntawm qhov ib txwm muaj. Tom qab tshem tawm cov kab mob, CRP poob qis thiab rov qab mus rau qhov ib txwm muaj hauv ib lub lis piam. Txawm li cas los xij, CRP tsis nce ntxiv ntau hauv cov ntaub ntawv ntawm kev kis tus kab mob viral, uas muab lub hauv paus rau kev txheeb xyuas cov kab mob thaum ntxov, thiab yog ib qho cuab yeej rau kev txheeb xyuas cov kab mob viral lossis bacterial.

TXOJ CAI NTAWM COV TXHEEJ TXHEEM

Lub membrane ntawm lub cuab yeej kuaj yog coated nrog anti CRP antibody rau ntawm thaj chaw kuaj thiab tshis anti luav IgG antibody rau ntawm thaj chaw tswj. Lub hau npog yog coated los ntawm fluorescence labeled anti CRP antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, CRP antigen hauv cov qauv ua ke nrog fluorescence labeled anti CRP antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv cov kev taw qhia ntawm daim ntawv nqus dej, thaum complex dhau thaj chaw kuaj, nws ua ke nrog anti CRP txheej antibody, tsim cov complex tshiab. CRP theem yog correlated zoo nrog fluorescence teeb liab, thiab qhov concentration ntawm CRP hauv cov qauv tuaj yeem pom los ntawm fluorescence immunoassay assay.

COV REAGENTS THIAB COV KHOOM SIV

Cov khoom siv pob khoom 25T：

Daim npav xeem ib tus zuj ib tus, ntim nrog lub desiccant 25T

Cov qauv tshuaj diluents 25T

Daim ntawv ntxig rau pob khoom 1

COV KHOOM SIV YUAV TSUM TAU TAB SIS TSIS TAU MUAB

Lub thawv sau qauv, lub sijhawm

KEV SAIB XYUAS THIAB CIA COV QAUV

1. Cov qauv kuaj tuaj yeem yog serum, heparin anticoagulant plasma lossis EDTA anticoagulant plasma.
2. Raws li cov txheej txheem txheem, sau cov qauv. Cov qauv ntshav lossis cov ntshav plasma tuaj yeem khaws cia rau hauv lub tub yees ntawm 2-8 ℃ rau 7 hnub thiab cryopreservation hauv qab -15 ° C rau 6 lub hlis. Cov qauv ntshav tag nrho tuaj yeem khaws cia rau hauv lub tub yees ntawm 2-8 ℃ rau 3 hnub
3. Tag nrho cov qauv zam kev khov-thaw voj voog.