Kev kuaj mob cov khoom siv rau 25-Hydroxy Vitamin D(fluorescence imtimochromatogromographic ensay)
Rau hauv vitro kuaj xyuas siv nkaus xwb
Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.

Siv
Kev kuaj mob cov khoom siv rau 25-hydroxy vitamin D (fluorescatosed immomotochromatographic assay) yog cov ntshav qab zib, uas yog siv los ntsuas cov qib Ntawm vitamin D.it yog ib qho kev ntsuas zauv uas yog ib qho kev kuaj mob reagent.all yuav tsum tau lees paub los ntawm lwm cov txheej txheem. Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb.

Lub ntsiab
Vitamin D yog vitamin thiab kuj tseem yog tshuaj steroid tshuaj, feem ntau yog suav nrog VD2 thiab VD3, uas nws txoj kev sib tw zoo heev. Vitamin D3 thiab D2 tau hloov dua siab tshiab txog 25 hydroxyl vitamin d (suav nrog 25-Dihydroxyl vitamin D3 thiab D2). 25- (Huag) VD hauv tib neeg lub cev, kev sib tsoo ruaj khov, siab siab. 25- (Oh) VD hais txog tag nrho cov vitamin D, thiab kev hloov dua siab tshiab ntawm Vitamin D, yog li 25- ((((Oh Immunochratographyographyographyographyog thiab tuaj yeem muab tau rau hauv 15 feeb.

Lub hauv paus ntsiab lus ntawm cov txheej txheem
Daim nyias nyias ntawm cov cuab yeej ntsuas yog coated nrog conjugate ntawm BSA thiab 25- (Oh) Luav thiab tshis Anti Lij igg antibody ntawm cheeb tsam tswj. Cim PAWKE TAW LOS NTAWM FORRORCCTS los ntawm Fluorescence Cim 1- (Oh) VD antibody thiab luav IGG ua ntej. Thaum kuaj cov qauv, 25- (Oh) VD nyob rau hauv cov qauv ua ke nrog fluorescence los tiv thaiv anti 25- (Oh) VD antibodody, thiab tsim kev tiv thaiv kev tiv thaiv kab mob. Nyob rau hauv qhov kev txiav txim ntawm immunochnumaphyography, cov complex ntws ntawm cov ntawv xeem, thaum muaj kev sib tw fluorescent yuav ua ke nrog membrane.Thaum siab ntawm 25- "(Oh) VD tsis zoo cuam tshuam rau fluorescence teeb liab, thiab kev xav tau zoo ntawm 25- (Oh) VD hauv cov qauv tuaj yeem raug kuaj pom ntawm fluorescence requay.

Reagents thiab khoom siv

25T Pob Cheebtsam:
.Test card card ib txwm foiled pouched nrog desiccant 25t
.A Kev daws 25T
.B daws 1
.Package ntxig rau 1

Cov ntaub ntawv yuav tsum tau tab sis tsis muab
Qauv sau ntim rau, timer

Qauv Sau thiab Cia
1.Qhov kev kuaj mob tuaj yeem yog ntshav, heparin anticoagulant ntshav lossis edta anticoagulant ntshav.
2.Txoj kev sib txuam cov qauv txheej txheem sau cov qauv. Ntshav dej lossis cov qauv ntshav lossis cov qauv ntshav lossis cov qauv ua tub rog tuaj yeem khaws cia hauv tub yees ntawm 2-8 ℃ rau 7days thiab cryoprimerve hauv qab -15 ° C rau 6 lub hlis.
3.Txhua kev coj tsis txhob ua kom tsis txhob khov-thaw cycles.

Txheej txheem assay
Cov txheej txheem ntsuas ntawm qhov ntsuas pom lub tshuab txhaj tshuaj Imunoanalyzer. Cov txheej txheem ntsuas ntsuas hluav taws xob yog raws li hauv qab no
1.Txhua txhua yam tshuaj tiv thaiv thiab cov hnoos qeev mus rau hnub sov.
2.Op Lub portable antalable artalyzer (Wiz-A101), nkag mus rau tus lej nyiaj nkag mus raws cov khoom lag luam, thiab nkag mus nrhiav qhov kev tshawb pom.
3.Can Cov kws tu hniav rau cov kws kho hniav kom paub meej tias cov khoom xeem.
4.Txhua daim npav xeem los ntawm lub hnab nyiaj.
5.insert daim npav xeem rau hauv daim npav qhov, scan tus QR code, thiab txiav txim siab cov khoom xeem.
6.Add 30μl Ntshav lossis Plasma coj mus rau ib qho kev daws teeb meem, thiab sib tov zoo.
7.DD 50μV B Cov kev daws teeb meem rau cov sib xyaw saum toj no, thiab sib tov zoo.
8.Tawm ntawm cov sib tov rau 15 feeb.
9.Qhov 80μl sib xyaw kom coj mus piv rau daim npav zoo.
10.Nyem "Tus Qauv Xeem Ntawv", tom qab 10 feeb, qhov ntsuas yuav cia li ntes tau daim ntawv xeem, thiab sau cov ntaub ntawv xeem.
11.Refer rau kev qhia ntawm lub portable ageler (wiz-a101).

Cia siab tias muaj txiaj ntsig
25- (Huag) VD cov khoom lag luam: 30-100ng / mL
Nws raug nquahu tias txhua lub chaw kuaj xyuas tsim nws tus kheej li qub uas nws tus neeg mob ib txwm muaj.

Kuaj cov qhabnias thiab kev txhais lus
.Qhov cov ntaub ntawv saum toj no yog cov ntaub ntawv sib nrug tsim rau cov ntaub ntawv kuaj pom ntawm cov khoom siv no, thiab nws tau qhia txog qhov tseem ceeb rau cov pej xeem muaj feem ntawm cov pej xeem hauv cheeb tsam no.
.Qhov concentration ntawm 25- (Oh) VD siab dua li cov kev siv, thiab kev hloov pauv ntawm kev ntxhov siab lossis kev tsis txaus ntseeg, yuav tsum paub txog kev kuaj mob.
Cov .Qhov ntawm cov qauv no tsuas yog siv rau cov ntaub ntawv siv tau tsim los ntawm cov qauv no, thiab cov txiaj ntsig tsis yog piv nrog lwm txoj kev.
Cov. Ua rau cov khoom siv tsis pom kev ua kom pom cov txiaj ntsig tau pom, suav nrog cov laj thawj, kev ua haujlwm thiab lwm yam qauv.

Cia thiab Ruaj Khwv
Cov .Qhov khoom siv yog 18 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis tau ntawm 2-30 ° C. Tsis khov. Tsis txhob siv dhau hnub tas sij hawm.
.Do tsis qhib lub hnab ntim khoom kom txog thaum koj tau siv xeem ua ib puag ncig (kub 2-35 ℃, av noo 40-90%) tsis pub dhau 60 mins sai li sai tau tau.
.Sample diluent yog siv tam sim ntawd tom qab qhib.

Ceeb Toom thiab Ceev Faj
Cov .Qhov khoom siv yuav tsum tau muab khi thiab tiv thaiv tiv thaiv noo noo.
.Txhua cov khoom zoo yuav tsum raug muab los ntawm lwm cov txheej txheem.
.All cov txheej txheem yuav raug kho kom zoo li muaj kuab paug.
.Do tsis siv reagent reagent.
.Do tsis tau sib pauv reagents ntawm cov khoom siv nrog sib txawv ntau tsis muaj ..
.Do tsis siv daim npav xeem thiab cov khoom siv pov tseg.
.Misoperation, ntau dhau los yog me me qauv tuaj yeem ua rau muaj kev cuam tshuam.

LKev qog
.As nrog ib qho zoo li cov tshuaj tua kab mob nas, qhov ua tau tshwm sim los ntawm tib neeg kev tawm tsam nas tua kab mob (HAMA) hauv qhov quav. Cov quav dev los ntawm cov neeg mob uas tau txais kev npaj ntawm monoclonhal tshuaj tiv thaiv rau kev kuaj mob lossis kev kho mob tuaj yeem muaj Hama. Cov hnoos qeev no yuav ua rau cov txiaj ntsig zoo lossis tsis tseeb.
.Qhov kev xeem ua tsuas yog rau kev kuaj mob, yuav tsum tsis txhob txiav txim siab ua ke nrog nws cov tsos mob hauv kev kho mob, kev kuaj mob, Epidemiology thiab lwm yam ntaub ntawv Cov.
.This reagent tsuas yog siv rau ntshav thiab ntshav cov kev ntsuam xyuas. Tej zaum nws yuav tsis tau txais qhov tshwm sim raug thaum siv rau lwm qhov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.

Cov yam ntxwv ua tau zoo

REFLERENY

1.Txhua kev cuam tshuam nrog Al.Hama cuam tshuam nrog Murine Monoclonal Antibodys [J].
2.Levinson s.Tus ntawm heterophilic antibodies thiab lub luag haujlwm hauv kev cuam tshuam ntawm immunoassay, 1992,15: 108-114.

Qhov tseem ceeb rau cov cim siv:

	Hauv cov vitro kuaj kho mob kho mob
	Tus tsim
	Khw ntawm 2-30 ℃
	Hnub tag sijhawm
	Tsis txhob rov siv dua
	Ceev faj
	Tham cov lus qhia kev siv

Xiamen Wiz Biotech CO., Ltd
Chaw Nyob: 3-4 pem teb, no.16 lub tsev, Rio-Medhoo Rhiav, 2030 Wengjiao West Txoj Kev, Lub Tsev Kawm Ntawv Hengeviao, 361026, Xiamen, Suav
Tel: + 86-592-6808278
Fax: + 86-592-6808279