Cov Khoom Siv Kuaj Mob rau 25-hydroxy Vitamin D(Kev ntsuam xyuas fluorescence immunochromatographic)
Rau kev siv kuaj mob hauv vitro xwb
Thov nyeem daim ntawv qhia no kom zoo ua ntej siv thiab ua raws li cov lus qhia. Kev ntseeg tau ntawm cov txiaj ntsig ntawm kev sim tsis tuaj yeem lav yog tias muaj kev hloov pauv ntawm cov lus qhia hauv daim ntawv qhia no.

YUAV TSUM SIV LI CAS
Diagnostic Kit rau 25-hydroxy Vitamin D (fluorescence immunochromatographic assay) yog ib tug fluorescence immunochromatographic assay rau qhov ntau ntawm 25-hydroxy Vitamin D (25-(OH)VD) nyob rau hauv tib neeg cov ntshav los yog ntshav, uas yog feem ntau yog siv los ntsuam xyuas cov theem ntawm vitamin D.Nws yog ib qho kev pab kuaj mob reagent.All zoo qauv yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev xeem no yog rau kev kho mob kev siv xwb.

LUS QHIA
Vitamin D yog ib qho vitamin thiab kuj yog ib qho tshuaj hormones steroid, feem ntau suav nrog VD2 thiab VD3, uas nws cov qauv zoo sib xws heev. Vitamin D3 thiab D2 raug hloov mus ua 25 hydroxyl vitamin D (suav nrog 25-dihydroxyl vitamin D3 thiab D2). 25-(OH) VD hauv tib neeg lub cev, kev tsim kho ruaj khov, muaj kev sib xyaw siab. 25-(OH) VD qhia txog tag nrho cov vitamin D, thiab lub peev xwm hloov pauv ntawm vitamin D, yog li 25-(OH) VD raug suav hais tias yog qhov qhia zoo tshaj plaws rau kev ntsuam xyuas qib ntawm vitamin D. Lub Khoom Siv Kuaj Mob yog raws li immunochromatography thiab tuaj yeem muab cov txiaj ntsig hauv 15 feeb.

TXOJ CAI NTAWM COV TXHEEJ TXHEEM
Lub membrane ntawm lub cuab yeej kuaj tau coated nrog conjugate ntawm BSA thiab 25-(OH)VD ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody ntawm thaj chaw tswj. Lub cim ncoo tau coated los ntawm fluorescence cim tiv thaiv 25-(OH)VD antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv, 25-(OH)VD hauv cov qauv ua ke nrog fluorescence cim tiv thaiv 25-(OH)VD antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv qhov kev taw qhia ntawm daim ntawv nqus, thaum complex dhau thaj chaw kuaj, Lub cim fluorescent dawb yuav raug ua ke nrog 25-(OH)VD ntawm daim nyias nyias. Qhov concentration ntawm 25-(OH)VD yog qhov tsis zoo rau lub teeb liab fluorescence, thiab qhov concentration ntawm 25-(OH)VD hauv cov qauv tuaj yeem pom los ntawm fluorescence immunoassay assay.

COV REAGENTS THIAB COV KHOOM SIV

Cov khoom siv pob khoom 25T：
.Test daim ntawv ib lub zuj zus ntawv ci pouched nrog ib tug desiccant 25T
.Ib qho kev daws teeb meem 25T
.B kev daws teeb meem 1
.Daim ntawv ntxig rau pob khoom 1

COV KHOOM SIV YUAV TSUM TAU TAB SIS TSIS TAU MUAB
Lub thawv sau qauv, lub sijhawm

KEV SAIB XYUAS THIAB CIA COV QAUV
1. Cov qauv kuaj tuaj yeem yog serum, heparin anticoagulant plasma lossis EDTA anticoagulant plasma.
2. Raws li cov txheej txheem txheem, sau cov qauv. Cov qauv ntshav lossis cov ntshav tuaj yeem khaws cia rau hauv lub tub yees ntawm 2-8 ℃ rau 7 hnub thiab cryopreservation hauv qab -15 ° C rau 6 lub hlis.
3. Tag nrho cov qauv tsis txhob khov thiab yaj.

TXHEEJ TXHEEM KEV NTSUAM XYUAS
Cov txheej txheem sim ntawm cov cuab yeej saib phau ntawv qhia immunoanalyzer. Cov txheej txheem sim reagent yog raws li nram no
1. Tso tag nrho cov tshuaj reagents thiab cov qauv rau qhov kub thiab txias hauv chav.
2. Qhib lub Portable Immune Analyzer (WIZ-A101), sau tus password nkag mus rau hauv tus account raws li txoj kev ua haujlwm ntawm lub cuab yeej, thiab nkag mus rau qhov interface nrhiav pom.
3. Luam theej daim code dentification kom paub meej tias yog yam khoom xeem.
4. Tshem daim ntawv xeem ntawm lub hnab ntawv ci.
5. Muab daim npav xeem tso rau hauv qhov chaw tso daim npav, luam theej daim QR code, thiab txiav txim siab seb yam khoom xeem yog dab tsi.
6. Ntxiv 30μL serum lossis plasma qauv rau hauv A kua, thiab sib tov kom zoo.
7. Ntxiv 50μL B kua rau hauv cov sib tov saum toj no, thiab sib tov kom zoo.
8.Cia cov sib tov rau 15 feeb.
9. Ntxiv 80μL sib tov rau hauv cov qauv zoo ntawm daim npav.
10. Nyem lub pob "kev xeem txheem", tom qab 10 feeb, lub cuab yeej yuav ntes tau daim npav xeem, nws tuaj yeem nyeem cov txiaj ntsig los ntawm qhov screen zaub ntawm lub cuab yeej, thiab sau / luam tawm cov txiaj ntsig xeem.
11. Saib cov lus qhia ntawm Portable Immune Analyzer (WIZ-A101).

TUS NQI XAV TAU
25-(OH)VD ib txwm muaj: 30-100ng/mL
Nws raug pom zoo kom txhua lub chaw kuaj mob tsim nws tus kheej qhov ntau thiab tsawg ib txwm sawv cev rau nws cov neeg mob.

COV TXIAJ NTSUAM XYUAS THIAB KEV TXHAIS LUS
. Cov ntaub ntawv saum toj no yog qhov siv tau ntev uas tau tsim los rau cov ntaub ntawv nrhiav pom ntawm cov khoom siv no, thiab nws tau hais tias txhua lub chaw kuaj mob yuav tsum tsim kom muaj qhov siv tau ntev rau qhov tseem ceeb ntawm cov pej xeem hauv thaj av no.
.Qhov concentration ntawm 25-(OH)VD yog siab dua li qhov siv tau, thiab cov kev hloov pauv ntawm lub cev lossis kev ntxhov siab yuav tsum raug tshem tawm. Tseeb tiag, yuav tsum muab cov tsos mob kuaj mob ua ke.
.Cov txiaj ntsig ntawm txoj kev no tsuas yog siv tau rau qhov ntau yam uas tau tsim los ntawm txoj kev no, thiab cov txiaj ntsig tsis sib piv ncaj qha nrog lwm txoj kev.
Lwm yam tseem tuaj yeem ua rau muaj qhov yuam kev hauv cov txiaj ntsig nrhiav tau, suav nrog cov laj thawj kev siv tshuab, kev ua haujlwm yuam kev thiab lwm yam piv txwv.

CIA THIAB RUAJ NTSEG
. Cov khoom siv no muaj 18 lub hlis txij li hnub tsim khoom. Khaws cov khoom siv tsis tau siv ntawm 2-30 ° C. TSIS TXHOB KHOV. Tsis txhob siv dhau hnub tas sij hawm.
.Tsis txhob qhib lub hnab kaw kom txog thaum koj npaj txhij los ua qhov kev sim, thiab qhov kev sim ib zaug siv tau raug pom zoo kom siv rau hauv qhov chaw xav tau (kub 2-35 ℃, av noo 40-90%) hauv 60 feeb sai li sai tau.
.Sample diluent siv tam sim ntawd tom qab qhib.

COV LUS CEEV FAIB THIAB COV KEV CEEB TOOM
. Cov khoom siv yuav tsum tau kaw thiab tiv thaiv kom tsis txhob muaj dej noo.
Txhua yam qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev.
Txhua yam qauv yuav tsum raug kho raws li cov kuab paug.
TSIS TXHOB siv cov tshuaj reagent uas tas sij hawm lawm.
.TSIS TXHOB pauv cov tshuaj reagents ntawm cov khoom siv nrog ntau yam tsis muaj.
TSIS TXHOB siv cov ntawv xeem thiab cov khoom siv pov tseg dua.
Kev ua haujlwm tsis raug, ntau dhau lossis me me tuaj yeem ua rau muaj kev hloov pauv ntawm cov txiaj ntsig.

LKEV UA YEES
Ib yam li kev sim siv cov tshuaj tua kab mob nas, qhov ua tau tias yuav muaj kev cuam tshuam los ntawm cov tshuaj tua kab mob nas tib neeg (HAMA) hauv cov qauv kuaj. Cov qauv kuaj los ntawm cov neeg mob uas tau txais kev npaj ntawm cov tshuaj monoclonal rau kev kuaj mob lossis kev kho mob yuav muaj HAMA. Cov qauv kuaj no yuav ua rau muaj qhov tshwm sim tsis zoo lossis tsis zoo.
.Qhov kev xeem no tsuas yog rau kev siv hauv kev kho mob xwb, yuav tsum tsis txhob ua lub hauv paus rau kev kuaj mob thiab kev kho mob xwb, kev tswj hwm kev kho mob ntawm cov neeg mob yuav tsum tau txiav txim siab ua ke nrog nws cov tsos mob, keeb kwm kho mob, lwm yam kev kuaj mob hauv chaw kuaj mob, kev kho mob teb, kev kis kab mob thiab lwm yam ntaub ntawv.
Cov tshuaj reagent no tsuas yog siv rau kev kuaj ntshav thiab ntshav xwb. Nws yuav tsis tau txais qhov tshwm sim tseeb thaum siv rau lwm cov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.

COV CIM TXOG KEV UA HAUJ LWM

RCOV CIM XAV TAU

1.Hansen JH, thiab lwm tus.HAMA Kev Cuam Tshuam nrog Murine Monoclonal Antibody-Based Immunoassays [J].J ntawm Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.Qhov Xwm Txheej ntawm Heterophilic Antibodies thiab Lub Luag Haujlwm hauv Immunoassay Interference [J].J ntawm Clin Immunoassay, 1992,15: 108-114.

Tus yuam sij rau cov cim siv:

	Cov Khoom Siv Kho Mob Hauv Vitro Diagnostic
	Chaw Tsim Khoom
	Khaws cia ntawm 2-30 ℃
	Hnub tas sij hawm
	Tsis txhob siv dua
	CEEB TOOM
	Saib Cov Lus Qhia Txog Kev Siv

Xiamen Wiz Biotech CO., LTD
Chaw Nyob: 3-4 Pem Teb, NO.16 Tsev, Bio-medical Rhiav, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Suav Teb
Xov tooj: + 86-592-6808278
Fax: + 86-592-6808279