Kev kuaj mob pob khoom (colloidal kub) rau calproteectin
Kev kuaj mob cov khoom siv(Colloidal Kub)rau calproteectin
Rau hauv vitro kuaj xyuas siv nkaus xwb
Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.
Siv
Kev kuaj mob cov khoom siv rau Calprotectin (Cal) yog yog ib qho kev txiav txim siab kub Qhov kev xeem no yog tshuaj ntsuam xyuas. Txhua tus qauv zoo yuav tsum tau lees paub los ntawm lwm cov txheej txheem. Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb. Lub caij no, qhov kev ntsuas no yog siv rau cov twj IVD, tsis xav tau ntxiv.
Lub ntsiab
Cal yog Heterodimer, uas yog muaj ntawm MRP 8 thiab mrp 14. Nws muaj nyob rau hauv neutrophils cyonuclear cell wee. Cal yog mob theem protein, nws muaj ib qho kev ua tau zoo ncua ntev li ib lub lim tiam hauv tib neeg faeces, nws yog txiav txim siab ua tus kab mob bucker. Cov khoom siv yog qhov yooj yim, kev kuaj pom kev pom tseeb, kuaj pom Cal nyob rau hauv tib neeg faeces, nws muaj kev kuaj pom siab thiab muaj zog tshwj xeeb. Qhov kev xeem raws li cov tshuaj tiv thaiv sandwich thiab kub immunomatogromogratogromogratogromogromographic thev naus laus zis, nws tuaj yeem muab rau hauv 15 feeb.
Lub hauv paus ntsiab lus ntawm cov txheej txheem
Cov Sawb muaj anti Cal txheej mcab ntawm cov cheeb tsam sim thiab tshis los ntawm membrane txoj kev ua haujlwm chromatography ua ntej. Lable Pad yog coated los ntawm colloidal kub sau npe anti Cal mcab thiab colloidal kub sau lowited luav ua ntej. Thaum kuaj xyuas cov qauv zoo, tus cal hauv cov qauv suav nrog cov kab mob sib tsoo, raws li tau tso cai rau kev tawm tsam los ntawm anti calating mcab ntawm daim nyias nyias thiab daim ntawv "Anti calating mcab-cal-colloidal kub sau npe anti Cal mcab" nyuaj, ib qho kev sim xim tau tshwm sim ntawm thaj av xeem. Cov xim siv xim yog qhov sib txheeb zoo nrog cov ntsiab lus. Ib qho qauv tsis zoo tsis tsim cov kev sim band vim qhov tsis muaj colloidal kub conjugate cal complex. Tsis muaj teeb meem Cal yog nyob rau hauv cov qauv lossis tsis yog, muaj ib thaj av liab tshwm sim nyob rau cheeb tsam thiab tswj hwm kev tswj hwm zoo, uas tau pom zoo ua cov qauv kev lag luam sab hauv.
Reagents thiab khoom siv
25T Pob Cheebtsam:
.Test card card alentually ntawv ci pouched nrog tus desiccant
.Sample Diluents: Cov khoom xyaw yog 20mm ph7.4PBs
.Dispette
.Package ntxig
Cov ntaub ntawv yuav tsum tau tab sis tsis muab
Qauv sau ntim rau, timer
Qauv Sau thiab Cia
Siv cov thawv ntim huv pov tseg kom khaws cov qauv dej tshiab, thiab kuaj tam sim ntawd. Yog tias tsis raug kuaj tam sim ntawd, thov khaws cia ntawm 2-8 ° C rau 12hours lossis cov bake -15 ° C rau 4 lub hlis.
Txheej txheem assay
1.Thaum cov qauv uas muaj cov qauv qhia, tom qab ntawd muab cov qauv qhia rov qab, rov ua kom nruj thiab ua kom zoo, rov ua qhov kev txiav txim 3 zaug. Los yog siv cov qauv uas lo tsis sib xws li 50mg faeces piv txwv, thiab muab tso rau hauv ib faeces qauv tube uas muaj cov qauv siv, thiab ntsia hlau nruj.
2. Siv cov pipette pov tseg coj tus neeg mob thinner, tom qab ntawd ntxiv 3 tus quav dej thiab maj mam muab tso rau ib sab.
3.Txoj tawm daim npav xeem los ntawm lub hnab nyiaj no, muab tso rau ntawm cov lus theem thiab khij nws.
4.Thaum lub hau los ntawm cov qauv hlua thiab muab pov tseg thawj ob drops diluted qauv ntsug thiab maj mam ua cov qauv zoo ntawm daim npav nrog muab cov khoom xa tawm, pib ncua sijhawm.
5.Qhov yuav tsum tau nyeem nyob rau hauv 10-15 feeb, thiab nws tsis yog tom qab 15 feeb.
Kuaj cov qhabnias thiab kev txhais lus
| Kuaj cov txiaj ntsig | Kev txhais lus | |
| ① | Red Siv Band thiab Tswj Xyuas Liab Bandappear ntawm R cheeb tsam thiab C cheeb tsam, tsis muaj xim liabKuaj band ntawm t cheeb tsam. | Nws txhais tau tias cov ntsiab lus ntawm tib neeg faecescalprotepotectin yog hauv qab [15μg / g, uas yog atheem. |
| ② | Red siv band thiab tswj kev tswj bandappear ntawm r cheeb tsam thiab C cheeb tsam, thiab covxim ntawm liab siv band yog tsaus duaKev kuaj tus pojniam liab. | Cov ntsiab lus ntawm tib neeg faeces calproteectin isbetween 15μg / g thiab 60μg / g. Uas yuav yognyob rau theem ib txwm, lossis tej zaum yuav muaj kev pheej hmoo ntawmChim siab plob platm syndrome. |
| ③ | Red siv band thiab tswj kev tswj bandappear ntawm r cheeb tsam thiab C cheeb tsam, thiab covxim ntawm liab siv band yog tib yam nrogKev kuaj tus pojniam liab. | Cov ntsiab lus ntawm tib neeg faeces calproteectin yog60 g, thiab muaj muaj kev pheej hmoo ntawmInars mob hnyuv. |
| ④ | Red siv band thiab tswj kev tswj bandappear ntawm r cheeb tsam thiab C cheeb tsam, thiab covxim ntawm liab xeem band yog tsaus dua liab duaKev Siv Pawg. | Nws qhia cov ntsiab lus ntawm tib neeg faecescalprotepotectin yog ntau dua 60μg / g, thiab muajyog kev pheej hmoo pheej hmoo ntawm mob plab hnyuvKab mob. |
| ⑤ | Yog hais tias liab siv band thiab tswj kev tswj hwm liab tsis pom lossis tsuas pom ib qho xwb, qhov kev xeem yogsuav hais tias tsis yog. | Rov ua qhov kev xeem siv daim npav xeem ntawv tshiab. |
Cia thiab Ruaj Khwv
Cov khoom siv yog 24 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis tau ntawm 2-30 ° C. Tsis txhob qhib lub hnab ntim khoom kom txog thaum koj npaj tau los ua qhov kev sim.
Ceeb Toom thiab Ceev Faj
1.Qhov khoom siv yuav tsum tau muab khi thiab pov hwm tiv thaiv ya raws1.
2.Tsis tsis txhob siv cov qauv uas tau muab tso rau ntev ntev lossis rov qab khov thiab yaj ntawm kev sim
3.FECAL cov qauv yog ntau dhau los yog tuab tuaj yeem ua cov qauv kev sib tw ua kom tiav, thov centrifuge cov qauv diluted thiab coj tus supernatant rau kev sim.
4.MisOperation, ntau dhau los lossis me me qauv tuaj yeem ua rau muaj kev cuam tshuam.
Kev txwv
1.Qhov kev xeem xeem ntawv tsuas yog siv rau kev kuaj mob, yuav tsum tsis txhob txiav txim siab ua ke rau nws cov tsos mob kev kuaj mob, kev kuaj mob, kho mob, Epidemiology thiab lwm yam lus qhia2Cov.
2.Qhov reagent tsuas yog siv rau kev kuaj quav. Tej zaum nws yuav tsis tau txais qhov tshwm sim raug thaum siv rau lwm qhov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.
Ntawv Sawv cev
[1] Cov Kws Kuaj Mob Hauv Tebchaws Hauv Tebchaws (cov txheej txheem thib peb, 2006) .the Ministry Health Department.
[2] Kev ntsuas rau kev tswj hwm ntawm cov kev kuaj mob vitro. Tuam Tshoj cov zaub mov noj thiab tshuaj, tsis muaj. 5 Kev txiav txim, 2014-07-30.
Qhov tseem ceeb rau cov cim siv:
 | Hauv cov vitro kuaj kho mob kho mob |
 | Tus tsim |
 | Khw ntawm 2-30 ℃ |
 | Hnub tag sijhawm |
 | Tsis txhob rov siv dua |
 | Ceev faj |
 | Tham cov lus qhia kev siv |
Xiamen Wiz Biotech CO., Ltd
Chaw Nyob: 3-4 pem teb, no.16 lub tsev, Rio-Medhoo Rhiav, 2030 Wengjiao West Txoj Kev, Lub Tsev Kawm Ntawv Hengeviao, 361026, Xiamen, Suav
Tel: + 86-592-6808278
Fax: + 86-592-6808279
