Diagnostic Kit(Colloidal Kub)rau Calprotectin
Rau kev kuaj mob hauv vitro nkaus xwb
Thov nyeem cov pob no kom zoo ua ntej siv thiab ua raws li cov lus qhia nruj me ntsis. Kev ntseeg siab ntawm cov txiaj ntsig kev ntsuas tsis tuaj yeem lav tau yog tias muaj kev sib txawv ntawm cov lus qhia hauv pob no.

YUAV TSUM SIV
Diagnostic Kit for Calprotectin (cal) yog ib tug colloidal kub immunochromatographic ntsuam xyuas rau ib tug semiquantitative kev txiav txim ntawm cal los ntawm tib neeg quav, uas muaj ib tug tseem ceeb accessory kev kuaj mob tus nqi rau inflammatory plob tsis so tswj kab mob. Qhov kev sim no yog kev tshuaj ntsuam reagent. Tag nrho cov qauv zoo yuav tsum tau lees paub los ntawm lwm cov txheej txheem. Qhov kev sim no yog npaj rau kev siv kws kho mob nkaus xwb. Lub caij no, qhov kev sim no yog siv rau IVD, tsis xav tau cov cuab yeej ntxiv.

TSEEM CEEB
Cal yog ib tug heterodimer, uas yog tsim los ntawm MRP 8 thiab MRP 14. Nws muaj nyob rau hauv neutrophils cytoplasm thiab qhia rau mononuclear cell membranes. Cal yog mob theem proteins, nws muaj ib tug ruaj khov theem txog ib lub lim tiam nyob rau hauv tib neeg faeces, nws txiav txim siab los ua ib tug inflammatory plob tsis so tswj kab mob. Cov khoom siv yog ib qho yooj yim, pom qhov kev ntsuam xyuas uas pom cal nyob rau hauv tib neeg faeces, nws muaj siab nrhiav tau rhiab heev thiab muaj zog tshwj xeeb. Qhov kev ntsuam xyuas raws li qhov tshwj xeeb ntawm ob chav tshuaj tiv thaiv kab mob Sandwich cov ntsiab lus thiab cov kev ntsuas kub immunochromatographic tshuaj ntsuam xyuas, nws tuaj yeem muab cov txiaj ntsig tsis pub dhau 15 feeb.

TSEV KAWM NTAWV NO
Cov sawb muaj cov tshuaj tiv thaiv cal txheej McAb ntawm thaj chaw kuaj thiab tshis los tiv thaiv luav IgG tshuaj tiv thaiv ntawm cheeb tsam tswj, uas yog fastened rau daim nyias nyias chromatography ua ntej. Lable pad yog coated los ntawm colloidal kub sau los tiv thaiv cal McAb thiab colloidal kub sau luav IgG antibody ua ntej. Thaum kuaj cov qauv zoo, cov cal nyob rau hauv cov qauv tuaj nrog colloidal kub sau los tiv thaiv cal McAb, thiab daim ntawv tiv thaiv kab mob, raws li nws tau tso cai rau tsiv teb tsaws raws li qhov kev xeem strip, cal conjugate complex yog ntes los ntawm anti cal txheej McAb ntawm daim nyias nyias thiab daim ntawv. "anti cal coating McAb-cal-colloidal gold labeled anti cal McAb" complex, ib pawg kuaj xim xim tau tshwm sim hauv cheeb tsam xeem. Cov xim siv tau zoo sib xws nrog cov ntsiab lus cal. Ib qho piv txwv tsis zoo tsis tsim ib qho kev sim qhab vim tsis muaj colloidal kub conjugate cal complex. Tsis muaj teeb meem cal yog tam sim no nyob rau hauv cov qauv los yog tsis, muaj ib tug liab kab txaij tshwm sim nyob rau hauv reference cheeb tsam thiab zoo tswj cheeb tsam, uas yog suav hais tias raws li zoo nyob rau hauv lub enterprise qauv.

REAGENTS THIAB KHOOM PLIG
25T pob khoom:
.Test card ib tus zuj zus daim ntawv ntim nrog lub desiccant
.Sample diluents: cov khoom xyaw yog 20mM pH7.4PBS
.Dispette
.Package insert

Cov khoom yuav tsum tau tab sis tsis muab

Sample ntim thawv, timer

SAMPLE COLLECTION THIAB STORAGE
Siv lub thawv huv huv pov tseg kom khaws cov faeces tshiab, thiab sim tam sim ntawd. Yog tias tsis tuaj yeem kuaj tam sim ntawd, thov khaws cia ntawm 2-8 ° C rau 12 teev lossis hauv qab -15 ° C rau 4 lub hlis.

TSEEM CEEB
1. Tshem tawm cov sampling stick, muab tso rau hauv cov qauv faeces, ces muab cov sampling stick rov qab, ntsia hlau nruj thiab co zoo, rov ua dua 3 zaug. Los yog siv cov sampling stickpicked txog 50mg faeces qauv, thiab muab tso rau hauv ib tug faeces qauv raj uas muaj cov qauv dilution, thiab ntswj kom nruj.
2. Siv cov khoom siv pov tseg pov tseg coj cov qauv faeces thinner los ntawm tus neeg mob raws plab, tom qab ntawd ntxiv 3 tee (kwv yees li 100uL) rau hauv lub raj fecal sampling tube thiab co zoo, muab tso tseg.
3. Tshem tawm daim ntawv xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.
4. Tshem lub hau ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 3 tee (li 100uL) tsis muaj npuas diluted qauv verticaly thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette, pib lub sijhawm.
5.Qhov tshwm sim yuav tsum tau nyeem nyob rau hauv 10-15 feeb, thiab nws yog invalid tom qab 15 feeb.

TEST RESULTS THIAB INTERPRETATION

KHOOM THIAB STABILITY
Cov khoom siv yog 24 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis siv ntawm 2-30 ° C. Tsis txhob qhib lub hnab kaw kom txog thaum koj npaj txhij ua qhov kev sim.
CEEB TOOM THIAB KEV TIV THAIV
1.Cov khoom siv yuav tsum tau muab kaw thiab tiv thaiv kom tsis txhob ya raws¹.
2.Tsis txhob siv cov qauv uas muab tso rau ntev dhau los lossis rov ua kom khov thiab thawing los kuaj
3.Fecal cov qauv ntau dhau los yog tuab tuaj yeem ua rau cov qauv diluted foul test card, thov centrifuge tus qauv diluted thiab coj tus supernatant mus kuaj.
4.Kev ua haujlwm tsis zoo, ntau dhau lossis cov qauv me me tuaj yeem ua rau muaj qhov sib txawv.

TXOJ CAI
1.Qhov kev ntsuam xyuas no tsuas yog siv rau kev soj ntsuam xwb, yuav tsum tsis txhob ua lub hauv paus rau kev kuaj mob thiab kev kho mob nkaus xwb, cov neeg mob kev tswj xyuas kev kho mob yuav tsum muaj kev txiav txim siab ua ke nrog nws cov tsos mob, keeb kwm kev kho mob, lwm yam kev kuaj mob, kev kho mob, kab mob sib kis thiab lwm yam. cov ntaub ntawv².
2.Qhov reagent no tsuas yog siv rau kev kuaj quav. Tej zaum nws yuav tsis tau txais txiaj ntsig zoo thaum siv rau lwm cov qauv xws li qaub ncaug thiab zis thiab lwm yam.