Cov Khoom Siv Kuaj Mob（Kub Colloidal)rau Calprotectin
Rau kev siv kuaj mob hauv vitro xwb
Thov nyeem daim ntawv qhia no kom zoo ua ntej siv thiab ua raws li cov lus qhia. Kev ntseeg tau ntawm cov txiaj ntsig ntawm kev sim tsis tuaj yeem lav yog tias muaj kev hloov pauv ntawm cov lus qhia hauv daim ntawv qhia no.

YUAV TSUM SIV LI CAS
Diagnostic Kit rau Calprotectin (cal) yog ib tug colloidal kub immunochromatographic assay rau semiquantitative kev txiav txim siab ntawm cal los ntawm tib neeg faeces, uas muaj ib qho tseem ceeb accessory diagnostic nqi rau inflammatory bowel kab mob. Qhov kev xeem no yog ib tug tshuaj ntsuam xyuas reagent. Tag nrho cov qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev xeem no yog rau kev kho mob cov kws kho mob siv xwb. Lub caij no, qhov kev xeem no yog siv rau IVD, ntxiv cov cuab yeej tsis xav tau.

LUS QHIA
Cal yog ib qho heterodimer, uas muaj MRP 8 thiab MRP 14. Nws muaj nyob rau hauv neutrophils cytoplasm thiab qhia tawm ntawm mononuclear cell membranes. Cal yog cov protein theem mob hnyav, nws muaj ib theem ruaj khov zoo li ib lub lim tiam hauv tib neeg cov quav, nws tau txiav txim siab tias yog ib qho cim qhia txog kab mob plab hnyuv. Lub pob khoom yog ib qho kev xeem yooj yim, pom tau semiqualitative uas ntes tau cal hauv tib neeg cov quav, nws muaj kev rhiab heev thiab muaj zog tshwj xeeb. Qhov kev xeem raws li kev tshwj xeeb siab ob chav antibodies sandwich reaction principle thiab kub immunochromatographic assay analysis technics, nws tuaj yeem muab cov txiaj ntsig hauv 15 feeb.

TXOJ CAI NTAWM COV TXHEEJ TXHEEM
Daim kab xev muaj txheej tiv thaiv cal McAb ntawm thaj chaw kuaj thiab cov tshuaj tiv thaiv tshis tiv thaiv luav IgG ntawm thaj chaw tswj, uas tau khi rau daim nyias nyias chromatography ua ntej. Daim ntawv lo yog coated los ntawm colloidal kub labeled anti cal McAb thiab colloidal kub labeled luav IgG antibody ua ntej. Thaum kuaj cov qauv zoo, cov cal hauv cov qauv sib xyaw nrog colloidal kub labeled anti cal McAb, thiab tsim cov tshuaj tiv thaiv kab mob, vim nws tau tso cai rau kev mus ncig raws daim kab xev kuaj, cov cal conjugate complex raug ntes los ntawm anti cal txheej McAb ntawm daim nyias nyias thiab tsim "anti cal txheej McAb-cal-colloidal kub labeled anti cal McAb" complex, ib daim kab xev kuaj xim tshwm sim ntawm thaj chaw kuaj. Qhov xim muaj feem cuam tshuam zoo nrog cov ntsiab lus cal. Cov qauv tsis zoo tsis tsim cov kab xev kuaj vim tsis muaj colloidal kub conjugate cal complex. Txawm tias cal muaj nyob hauv cov qauv lossis tsis muaj, muaj ib daim kab xev liab tshwm sim ntawm thaj chaw siv thiab thaj chaw tswj xyuas zoo, uas suav tias yog cov qauv kev lag luam zoo hauv tuam txhab.

COV REAGENTS THIAB COV KHOOM SIV
Cov khoom siv pob khoom 25T：
.Test daim ntawv ib tus zuj ib lub ntawv ci pouched nrog ib tug desiccant
.Cov qauv tshuaj diluents: cov khoom xyaw yog 20mM pH7.4PBS
.Dispette
.Ntxig pob khoom

COV KHOOM SIV YUAV TSUM TAU TAB SIS TSIS TAU MUAB

Lub thawv sau cov qauv, lub sijhawm

KEV SAIB XYUAS THIAB CIA COV QAUV
Siv lub thawv huv uas siv ib zaug xwb los sau cov quav tshiab, thiab kuaj tam sim ntawd. Yog tias kuaj tsis tau tam sim ntawd, thov khaws cia rau ntawm 2-8°C rau 12 teev lossis qis dua -15°C rau 4 lub hlis.

TXHEEJ TXHEEM KEV NTSUAM XYUAS
1. Muab tus pas ntsuas uas muab ntxig rau hauv cov quav kuaj, ces muab tus pas ntsuas rov qab, ntsia kom nruj thiab co kom zoo, rov ua qhov no 3 zaug. Los yog siv tus pas ntsuas uas muab kwv yees li 50mg cov quav kuaj, thiab muab tso rau hauv lub raj ntsuas cov quav uas muaj cov tshuaj ntxiv rau, thiab ntsia kom nruj.
2. Siv cov raj xa dej pov tseg los kuaj cov quav uas nyias dua los ntawm tus neeg mob raws plab, tom qab ntawd ntxiv 3 tee (kwv yees li 100uL) rau hauv lub raj kuaj quav thiab co kom zoo, muab tso rau ib sab.
3. Tshem daim ntawv xeem ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj sib dhos thiab cim nws.
4. Tshem lub hau ntawm lub raj xa dej thiab pov tseg thawj ob tee dej diluted qauv, ntxiv 3 tee dej (kwv yees li 100uL) tsis muaj npuas diluted qauv verticaly thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette, pib lub sijhawm.
5. Cov txiaj ntsig yuav tsum tau nyeem tsis pub dhau 10-15 feeb, thiab nws tsis raug cai tom qab 15 feeb.

COV TXIAJ NTSUAM XYUAS THIAB KEV TXHAIS LUS

CIA THIAB RUAJ NTSEG
Cov khoom siv no muaj peev xwm siv tau 24 lub hlis txij li hnub tsim khoom. Khaws cov khoom siv tsis tau siv rau ntawm 2-30 ° C. Tsis txhob qhib lub hnab kaw kom txog thaum koj npaj txhij los sim.
COV LUS CEEV FAIB THIAB COV KEV CEEB TOOM
1. Cov khoom siv yuav tsum tau kaw thiab tiv thaiv kom tsis txhob muaj dej noo¹.
2. Tsis txhob siv cov qauv uas muab tso ntev dhau los yog rov ua kom khov thiab yaj dua los sim.
3. Cov qauv quav ntau dhau los yog tuab tuaj yeem ua rau cov qauv diluted tsis zoo, thov centrifuge cov qauv diluted thiab coj cov supernatant mus kuaj.
4. Kev ua haujlwm tsis raug, ntau dhau lossis tsawg tus qauv tuaj yeem ua rau muaj kev hloov pauv ntawm cov txiaj ntsig.

TXWV
1. Cov txiaj ntsig ntawm kev xeem no tsuas yog siv rau kev siv hauv kev kho mob xwb, yuav tsum tsis txhob siv ua lub hauv paus rau kev kuaj mob thiab kev kho mob xwb, kev tswj hwm kev kho mob ntawm cov neeg mob yuav tsum tau txiav txim siab ua ke nrog nws cov tsos mob, keeb kwm kev kho mob, lwm yam kev kuaj mob hauv chaw kuaj mob, kev teb rau kev kho mob, kev sib kis kab mob thiab lwm yam ntaub ntawv.².
2. Cov tshuaj reagent no tsuas yog siv rau kev kuaj quav xwb. Nws yuav tsis tau txais qhov tshwm sim tseeb thaum siv rau lwm cov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.