Cov Khoom Siv Kuaj Mob rau Procalcitonin

(Kev ntsuam xyuas fluorescence immunochromatographic)

Rau kev siv kuaj mob hauv vitro xwb

Thov nyeem daim ntawv qhia no kom zoo ua ntej siv thiab ua raws li cov lus qhia. Kev ntseeg tau ntawm cov txiaj ntsig ntawm kev sim tsis tuaj yeem lav yog tias muaj kev hloov pauv ntawm cov lus qhia hauv daim ntawv qhia no.

YUAV TSUM SIV LI CAS

Kev Tshuaj Xyuas Khoom Siv rau Procalcitonin (fluorescence immunochromatographic assay) yog ib qho kev tshuaj xyuas fluorescence immunochromatographic rau kev kuaj pom ntau ntawm Procalcitonin (PCT) hauv tib neeg cov ntshav lossis cov ntshav, nws yog siv rau kev kuaj mob pab ntawm cov kab mob thiab sepsis. Tag nrho cov qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev xeem no yog rau kev siv kho mob xwb.

LUS QHIA

Procalcitonin muaj 116 hom amino acids thiab nws qhov hnyav molecular yog 12.7KD. PCT yog qhia los ntawm cov hlwb neuroendocrine thiab tawg los ntawm cov enzymes ua (tsis paub tab) calcitonin, carboxy-terminating peptide, thiab amino terminating peptide. Cov neeg noj qab haus huv tsuas muaj me ntsis PCT hauv lawv cov ntshav, uas tuaj yeem nce ntxiv tom qab kis kab mob. Thaum sepsis tshwm sim hauv lub cev, feem ntau cov ntaub so ntswg tuaj yeem qhia PCT, yog li PCT tuaj yeem siv ua qhov qhia txog kev mob sepsis. Rau qee tus neeg mob uas muaj kab mob inflammatory, PCT tuaj yeem siv ua qhov qhia txog kev xaiv tshuaj tua kab mob thiab kev txiav txim siab txog kev ua haujlwm zoo.

TXOJ CAI NTAWM COV TXHEEJ TXHEEM

Lub membrane ntawm lub cuab yeej kuaj yog coated nrog anti PCT antibody rau ntawm thaj chaw kuaj thiab tshis anti luav IgG antibody rau ntawm thaj chaw tswj. Lub hau npog yog coated los ntawm fluorescence labeled anti PCT antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, PCT antigen hauv cov qauv ua ke nrog fluorescence labeled anti PCT antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv cov kev taw qhia ntawm daim ntawv nqus dej, thaum complex dhau thaj chaw kuaj, nws ua ke nrog anti PCT txheej antibody, tsim cov complex tshiab. PCT theem yog correlated zoo nrog fluorescence teeb liab, thiab qhov concentration ntawm PCT hauv cov qauv tuaj yeem pom los ntawm fluorescence immunoassay assay.