PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog Cpn-IgM tshuaj tiv thaiv kab mob ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG tshuaj tiv thaiv ntawm cheeb tsam tswj. Lable ncoo yog coated los ntawm fluorescence sau los tiv thaiv Cpn-IgM antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, Cpn-IgM antigen hauv cov qauv ua ke nrog cov tshuaj fluorescence sau los tiv thaiv Cpn-IgM antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev txiav txim ntawm immunochromatography, complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv. Thaum complex dhau qhov kev xeem cheeb tsam, nws ua ke nrog anti Cpn-IgM txheej antibody, tsim tshiab complex. Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem

WIZ-A202 cov txheej txheem xeem thiab Portable Immune analyzer WIZ-A101 xa mus rau cov lus qhia ntawm kev qhia. Visual test txheej txheem yog raws li nram no:

1. Tshem tawm daim ntawv xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.
2. Ntxiv 10μl cov ntshav los yog cov ntshav plasma los yog 20ul tag nrho cov ntshav coj mus kuaj zoo ntawm daim npav nrog muab dispette, tom qab ntawd ntxiv 100μl (txog 2-3 poob) cov qauv diluent; pib lub sij hawm.
3. Tos kom tsawg kawg 10-15 feeb thiab nyeem qhov tshwm sim, qhov tshwm sim tsis raug tom qab 15 feeb.