TSAB NTAWV CAI

Lub membrane ntawm lub cuab yeej kuaj yog coated nrog Tf antibody rau ntawm thaj chaw kuaj thiab tshis anti luav IgG antibody rau ntawm thaj chaw tswj. Lub hau npog yog coated los ntawm fluorescence labeled anti Tf antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, Tf antigen hauv cov qauv ua ke nrog fluorescence labeled anti Tf antibody, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov complex ntws hauv kev coj ntawm daim ntawv nqus dej. Thaum complex dhau thaj chaw kuaj, nws ua ke nrog anti Tf txheej antibody, tsim cov complex tshiab.

Yog tias nws tsis zoo, cov qauv tsis muaj transferrin lossis cov ntsiab lus qis, yog li ntawd cov tshuaj tiv thaiv kab mob tsis tuaj yeem tsim tau. Yuav tsis muaj kab liab hauv thaj chaw kuaj pom (T). Txawm hais tias Tf antibody muaj nyob hauv cov qauv lossis tsis muaj, cov tshuaj tiv thaiv nas anti-human IgM monoclonal antibody uas muaj kub colloidal thiab cov tshuaj tiv thaiv tshis anti-nas IgG uas tau coated hauv thaj chaw tswj xyuas zoo (C) kab. Tom qab ntawd cov agglutinates tsim xim hauv thaj chaw tswj xyuas zoo, thiab kab liab yuav tshwm sim hauv (C). Kab liab yog tus qauv tshwm sim hauv thaj chaw tswj xyuas zoo (C) rau kev txiav txim siab seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas yog ib txwm muaj. Nws kuj tseem siv ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov Txheej Txheem Xeem

Thov nyeem daim ntawv qhia ua ntej sim.

1. Tshem daim ntawv xeem ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj sib dhos thiab cim nws.

2. Tshem lub hau ntawm lub raj xa dej thiab pov tseg ob tee dej uas tau diluted ua ntej, ntxiv 3 tee dej (kwv yees li 100uL) uas tsis muaj npuas diluted ua ntej thiab maj mam muab tso rau hauv qhov dej ntawm daim npav nrog lub dispette uas muab. Tom qab ntawd pib lub timer.