PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog Tf antibody ntawm thaj tsam kuaj thiab tshis anti luav IgG antibody ntawm cheeb tsam tswj. Lable ncoo yog coated los ntawm fluorescence sau los tiv thaiv Tf antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, Tf antigen hauv cov qauv ua ke nrog cov tshuaj fluorescence sau los tiv thaiv Tf antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev txiav txim ntawm immunochromatography, complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv. Thaum complex dhau qhov kev xeem cheeb tsam, nws ua ke nrog anti Tf txheej antibody, tsim tshiab complex.

Yog tias nws tsis zoo, cov qauv tsis muaj cov tshuaj hloov pauv lossis cov ntsiab lus qis, yog li tsis tuaj yeem tsim cov tshuaj tiv thaiv kab mob. Yuav tsis muaj kab liab nyob rau hauv qhov chaw kuaj pom (T). Txawm hais tias Tf antibody muaj nyob rau hauv cov qauv los yog tsis, qhov seem ntawm colloidal kub-labeled nas anti-tib neeg IgM monoclonal antibody thiab tshis anti-nas IgG antibody coated nyob rau hauv kev tswj qhov zoo (C) kab. Tom qab ntawd cov agglutinates tsim cov xim hauv cheeb tsam tswj kom zoo, thiab cov kab liab yuav tshwm hauv (C). Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem

Thov nyeem cov pob ntxig ua ntej kuaj.

1. Tshem tawm daim npav xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.

2. Tshem lub hau los ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 3 tee (li 100uL) tsis muaj npuas diluted qauv vertically thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette. Ces pib lub timer.