PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog Rotavirus Group A antigen ntawm thaj tsam kuaj thiab tshis anti luav IgG antibody ntawm thaj tsam tswj. Lable pad yog coated los ntawm fluorescence sau npe anti Rotavirus Group A thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, RV hauv cov qauv ua ke nrog cov tshuaj fluorescence sau los tiv thaiv Rotavirus Group A, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev txiav txim ntawm immunochromatography, complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv. Thaum complex dhau qhov kev xeem cheeb tsam, nws ua ke nrog anti-Rotavirus Group A txheej antibody, tsim tshiab complex. Yog tias nws tsis zoo, tsis muaj Rotavirus Group A antigen hauv cov qauv, yog li tsis tuaj yeem tsim cov tshuaj tiv thaiv kab mob, yuav tsis muaj kab liab hauv cheeb tsam kuaj pom (T). Txawm hais tias pab pawg A rotavirus muaj nyob rau hauv cov qauv, cov latex-labeled nas IgG yog chromatographed rau thaj tsam tswj zoo (C) thiab ntes los ntawm tshis anti-nas IgG antibody. Ib kab liab yuav tshwm sim hauv cheeb tsam tswj kom zoo (C). Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem:

1.Symptomatic cov neeg mob yuav tsum tau sau. Raws li cov lus ceeb toom, qhov siab tshaj plaws excretion ntawm rotavirus nyob rau hauv lub plab ntawm cov neeg mob gastroenteritis tshwm sim 3-5 hnub tom qab pib ntawm tus kab mob thiab 3-13 hnub tom qab pib cov tsos mob. Yog tias cov qauv sau tau ntev tom qab zawv plab, tus naj npawb ntawm antigens yuav tsis txaus los tshwm sim cov tshuaj tiv thaiv zoo.

2.Cov qauv yuav tsum tau muab sau rau hauv lub thawv huv, qhuav, tsis muaj dej tsis muaj cov tshuaj ntxuav tes thiab cov tshuaj tua kab mob.

3.Rau cov neeg mob uas tsis mob raws plab, cov qauv faeces khaws tseg yuav tsum tsis pub tsawg dua 1-2 grams. Rau cov neeg mob raws plab, yog tias lub plab yog kua, thov sau tsawg kawg 1-2 ml ntawm cov kua quav. Yog hais tias lub plab muaj cov ntshav thiab cov hnoos qeev ntau, thov sau cov qauv ntxiv.

4.Nws raug nquahu kom kuaj cov qauv tam sim tom qab sau, txwv tsis pub lawv yuav tsum raug xa mus rau chav kuaj hauv 6 teev thiab khaws cia ntawm 2-8 ° C. Yog tias cov qauv tsis tau kuaj hauv 72 teev, lawv yuav tsum tau muab khaws cia ntawm qhov kub ntawm -15 ° C.

5.Siv cov quav tshiab rau kev sim, thiab cov qauv faeces sib xyaw nrog cov dej diluent lossis distilled