PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog microalbumin antigen ntawm thaj tsam kuaj thiab tshis anti luav IgG antibody ntawm cheeb tsam tswj. Lable ncoo yog coated los ntawm fluorescence sau npe microalbumin thiab luav IgG ua ntej. Yog tias tsis muaj albumin nyob rau hauv cov zis, cov colloidal kub-labeled anti-Alb-labeled monoclonal antibody ntawm daim ntawv kub colloidal yuav khiav ntawm daim nyias nyias nrog cov zis mus rau txoj kab kuaj pom, thiab ua ke nrog Alb-coated antigen nrog pom. kab. Thiab cov xim kab yog tsaus dua li kab xim hauv cheeb tsam tswj (C), qhov no yog qhov tshwm sim tsis zoo. Yog tias cov zis muaj albumin, lawv yuav sib tw nrog Alb-coated antigen ntawm daim nyias nyias los khi rau qhov txwv cov tshuaj tiv thaiv kab mob ntawm cov colloidal kub-labeled anti-Alb-labeled monoclonal antibody. Raws li tus nqi ntawm albumin hauv cov zis nce, kuaj

Cov xim ntawm kab yuav dhau los ua sib dua thiab sib dua. Cov ntsiab lus ntawm albumin hauv cov zis tuaj yeem kuaj pom ib nrab ntawm qhov ntau los ntawm kev sib piv qhov kuaj pom (T) nrog thaj tsam tswj (C). Qhov chaw tswj xyuas qhov zoo (C) thiab thaj chaw siv (R) ntawm cov khoom siv yuav ib txwm tshwm sim thaum lub sijhawm kuaj, thiab tsis muaj dab tsi ua rau muaj cov zis albumin. Cov cheeb tsam tswj (C) thiab thaj tsam siv (R) kab tuaj yeem siv los ua qhov ntsuas kev tswj hwm sab hauv zoo rau cov khoom siv.

Cov txheej txheem xeem:

Thov nyeem phau ntawv ntsuas kev ua haujlwm thiab pob ntim ua ntej kuaj. Thaw cov qauv mus rau chav sov ua ntej siv.

1. Tshem tawm daim ntawv xeem los ntawm lub hnab ntawv ci. Muab nws ncaj nraim rau ntawm kab rov tav thiab cim.

2. Nqa cov zis nrog ib lub pipette pov tseg, muab pov tseg thawj ob tee ntawm cov zis coj mus kuaj. Ntxiv 3 tee (kwv yees li 100uL) ntawm cov zis tsis muaj npuas mus rau qhov nruab nrab ntawm qhov piv txwv ntawm daim npav vertically thiab pib lub sijhawm.

3.Nyeem qhov tshwm sim hauv 10-15 feeb. Invalid yog tias ntau tshaj 15 feeb.