PRINCIPLE

The membrane of the test device is coated with anti HbA1c antibody on the test region and anti Hb antibody on the control region. Lable pad are coated by fluorescence labeled anti Hb antibody and anti HbA1c antibody in advance. When testing  sample, the HbA1c antigen in sample combine with fluorescence labeled anti HbA1c antibody, the Hb antigen in sample combine with fluorescence labeled anti Hb antibodyand form immune mixture. Under the action of the immunochromatography, the complex flow in the direction of absorbent paper, when complex passed the test region, it combined with anti HbA1c coating antibody, forms new complex. when complex passed the control region, it combined with anti Hb coating antibody, forms new complex.HbA1c level is positively correlated with fluorescence signal, and the concentration of HbA1c and Hb in sample can be detected by fluorescence immunoassay assay.

Test Procedure:

Please read the instrument operation manual and package insert before testing.

1. Lay aside all reagents and samples to room temperature.
2. Open the Portable Immune Analyzer(WIZ-A101), enter the account password login according to the operation method of the instrument, and enter the detection interface.
3. Scan the dentification code to confirm the test item.
4. Take out the test card from the foil bag.
5. Insert the test card into the card slot, scan the QR code, and determine the test item
6. Add 10μL Whole blood into sample diluent, shake 1 min and mix well.
7. Add 80μL sample solution to sample well of the card.
8. Click the "standard test" button, after 15 minutes, the instrument will automatically detect the test card, it can read the results from the display screen of the instrument, and record/print the test results.
9. Refer to the instruction of Portable Immune Analyzer(WIZ-A101).