Hoʻokahi kapuaʻi kit wikiwiki rotavirus Group a me Adenovirus latex
Nā ʻāpana huahana
KUMU A ME KA HANA O KA FOB HO'O
KUMU
Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka hui A a me ka antigen adenovirus ma ka ʻāina hoʻāʻo a me ka antibody anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia ʻo anti Group A a me ka adenovirus a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi no ka Pūʻulu A a me ka adenovirus, hui pū ka Pūʻulu A a me ka adenovirus i ka laʻana me ka fluorescence i kapa ʻia ʻo anti Rotavirus Group A a me ka adenovirus, a hana i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, kahe ka paʻakikī i ke kuhikuhi o ka pepa absorbent. I ka hala ʻana o ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū me ka anti-Rotavirus Group A a me ka adenovirus coating antibody, e hana hou i kahi paʻakikī. Inā maikaʻi ʻole, ʻaʻohe Rotavirus Group A a me adenovirus antigen i loko o ka hāpana, no laila ʻaʻole hiki ke hoʻokumu ʻia nā paʻakikī immune, ʻaʻohe laina ʻulaʻula ma ka wahi ʻike (T). ʻO ka hui A rotavirus a me ka adenovirus i loko o ka specimen, ua chromatographed ka ʻiole IgG i kapa ʻia i ka latex i ka wahi hoʻomalu maikaʻi (C) a hopu ʻia e ka anti-mouse IgG antibody. E ʻike ʻia kahi laina ʻulaʻula ma ka wahi hoʻomalu maikaʻi (C). ʻO ka laina ʻulaʻula ka maʻamau i hōʻike ʻia ma ka ʻāpana hoʻomalu maikaʻi (C) no ka hoʻoholo ʻana inā lawa nā laʻana a inā he maʻamau ke kaʻina chromatography. Hoʻohana ʻia ia ma ke ʻano he maʻamau mana kūloko no nā reagents.
Kaʻina hana hoʻāʻo:
1. E wehe i ka pāpale o ka paipu hōʻiliʻili hāpana. Mai hoʻokahe i ka hopena i loko o ka'ōmole.
2. Wehe i ka lāʻau laʻau, hoʻokomo ʻia i loko o ka hāpana faeces (a i ʻole e hoʻohana i kahi lāʻau laʻau e ʻohi ai ma kahi o 50mg o nā feces), a laila e hoʻihoʻi i ka lāʻau laʻau, wili a lulu maikaʻi, e hana hou i ka hana 3 manawa. E lawe i kahi ʻāpana ʻokoʻa o ka hāpana faeces i kēlā me kēia manawa. Ma hope o ka laʻana, e hoʻokomo i ke koʻokoʻo laʻana i loko o ka pahu hōʻiliʻili faeces i loaʻa ka mea hoʻoheheʻe hāpana, a wili a paʻa i ka dropper. Inā ʻoi aku ka lahilahi o nā feces o ka mea maʻi me ka maʻi ʻaʻai, hiki ke hoʻohana ʻia kahi mauʻu palaka i hoʻohana ʻia no ka laʻana. Me ka hoʻohana ʻana i ka laʻana pipette hoʻokuʻu ʻia e lawe i ka laʻana o nā feces ʻoi aku ka lahilahi mai ka mea maʻi maʻi maʻi maʻi, a laila hoʻohui i 3 kulu (ma kahi o 100uL) i ka paipu hoʻoheheʻe fecal.
3. E lulu maikaʻi i ka hāpana a wehe i ka pāpale ma ka ʻaoʻao dropper a laila waiho ʻia.
4. Ke mālama ʻia i ka haʻahaʻa haʻahaʻa, pono e hoʻihoʻi ʻia ka pahu i ka lumi wela ma mua o ka hoʻohana ʻana. Wehe i ke kāleka hoʻāʻo mai ka ʻeke pepa, kau ma luna o ka pākaukau pae a kaha.
5. Wehe i ka pāpale mai ka hāpana paipu a hoolei i ka mua elua kulu diluted hāpana, hoʻohui i 3 kulu (e pili ana i 100uL) no bubble diluted hāpana vertically a me ka mālie i ka laʻana luawai o ke kāleka me ka hoʻolako dispette, hoʻomaka manawa.
6. Pono e heluhelu ʻia ka hopena i loko o 10-15 mau minuke, a ʻaʻole kūpono ia ma hope o 15 mau minuke.
E pili ana iā mākou
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