IgM antibody Enterovirus 71 EV71 pahu ho'āʻo wikiwiki EV 71 antibody
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KUMU A ME KA HANA O KA FOB HO'O
KUMU
Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka antibody anti EV71 ma ka ʻāina hoʻāʻo a me ke kao anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia i ka anti EV71 antibody a me ka rabbit IgG ma mua. Ke hoʻāʻo ʻana i ka laʻana maikaʻi, hui pū ka antigen EV71 i ka laʻana me ka fluorescence i kapa ʻia ʻo antibody EV71 antibody, a hana i ka hui kūʻokoʻa. Ma lalo o ka hana o ka chromatography, ke kahe paʻakikī i ke ala o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū me ka anti EV71 coating antibody, hana hou i ka paʻakikī.
Inā maikaʻi ʻole, ʻaʻole i loaʻa i ka hāpana enterovirus 71 IgM antibody, no laila ʻaʻole hiki ke hoʻokumu ʻia ka paʻakikī immune. ʻAʻohe laina ʻulaʻula ma ka wahi ʻike (T). ʻAʻohe mea pili i ka Enterovirus 71 IgM antibody i loko o ka specimen a i ʻole, ʻo ke koena colloidal gold-labeled mouse anti-human IgM monoclonal antibody a me ke kao anti-mouse IgG antibody i uhi ʻia ma ka wahi hoʻomalu maikaʻi (C). A laila hoʻomohala nā agglutinates i ke kala ma ka ʻāpana hoʻomalu maikaʻi, a ʻike ʻia ka laina ʻulaʻula ma (C). ʻO ka laina ʻulaʻula ka maʻamau i hōʻike ʻia ma ka ʻāpana hoʻomalu maikaʻi (C) no ka hoʻoholo ʻana inā lawa nā laʻana a inā he maʻamau ke kaʻina chromatography. Hoʻohana ʻia ia ma ke ʻano he maʻamau mana kūloko no nā reagents.
Kaʻina hana hoʻāʻo:
1. ʻO nā mea hoʻohālike i hoʻāʻo ʻia he koko holoʻokoʻa, me ke koko venous a i ʻole ke koko Peripheral. ʻAʻole hiki ke mālama ʻia ke koko holoʻokoʻa ma hope o ka ʻohi ʻana. Pono wau e hoʻohana koke ma hope o ka ʻohi ʻana.
2.ʻOhiʻia nā hōʻailona Serum aseptically e like me nāʻenehana maʻamau. ʻAʻole hiki ke hoʻohana i ka serum wela-inactivated. ʻAʻole ʻōlelo ʻia e hoʻohana i ka lipemic, turbid a contaminated serum. ʻO nā mea ʻāpana i ka serum. A e hoʻopilikia ka ua i nā hopena hoʻāʻo, pono e centrifuged a kānana ʻia kēlā mau laʻana ma mua o ka hoʻohana ʻana.
3. ʻO nā mea hoʻohālike i ho'āʻoʻia he heparin, Sodium citrate a iʻole EDTA anticoagulant plasma.
4.According i nā hana maʻamau e hōʻiliʻili i ka hāpana. Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 3 mau lā a me ka cryopreservation ma lalo o -15°C no 3 mahina.
5.All laʻana e pale i nā pōʻaiapuni freeze-thaw.
E pili ana iā mākou
ʻO Xiamen Baysen Medical Tech Limited kahi ʻoihana olaola kiʻekiʻe e hāʻawi iā ia iho i ka waiho ʻana i ka reagent diagnostic wikiwiki a hoʻohui i ka noiʻi a me ka hoʻomohala ʻana, ka hana ʻana a me ke kūʻai aku i kahi holoʻokoʻa. Nui nā limahana noiʻi kiʻekiʻe a me nā luna kūʻai aku i ka ʻoihana, loaʻa iā lākou āpau ka ʻike hana hana ma Kina a me nā ʻoihana biopharmaceutical honua.