ʻAʻohe mea kūʻai hou a i ʻole mea kūʻai aku kahiko, manaʻoʻiʻo mākou i ka ʻōlelo lōʻihi a me ka pilina hilinaʻi no Hot Sale FactoryKina Bvdv Hoao ʻO ka maʻi maʻi maʻi pipiʻO ka Virus Antibody Rapid Diagnostic Test, Ua mākaukau mākou e hāʻawi iā ʻoe i nā manaʻo maikaʻi loa i nā hoʻolālā o kāu mau kauoha ma ke ʻano ʻoihana inā pono ʻoe. I kēia manawa, ke hoʻomau nei mākou i ka hoʻomohala ʻana i nā ʻenehana hou a me ka hana ʻana i nā hoʻolālā hou i hiki ai iā ʻoe ke holomua ma ka laina o kēia ʻoihana.
ʻAʻohe mea kūʻai hou a i ʻole mea kūʻai aku kahiko, ke manaʻoʻiʻo nei mākou i ka ʻōlelo lōʻihi a me ka pilina hilinaʻi noʻO ka maʻi maʻi maʻi pipi, Kina Bvdv Hoao, Loaʻa iā mākou nā ʻenehana kiʻekiʻe ma kēia mau ʻoihana a me kahi hui kūpono i ka noiʻi. He aha hou aʻe, i kēia manawa ua loaʻa iā mākou kā mākou waihona waihona ponoʻī a me nā mākeke ma Kina ma ke kumu kūʻai haʻahaʻa. No laila, hiki iā mākou ke hālāwai i nā nīnau like ʻole mai nā mea kūʻai aku. E hoʻomanaʻo e ʻimi i kā mākou pūnaewele e nānā i ka ʻike hou aku mai kā mākou mea kūʻai.
ʻO ka pahu diagnostic no Estradiol(ka ho'āʻo immunochromatographic fluorescence)
No ka hoʻohana diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA
ʻO ka Diagnostic Kit no Estradiol (fluorescence immunochromatographic assay) he hōʻike immunochromatographic fluorescence no ka ʻike nui ʻana o Estradiol (E2) i loko o ka serum kanaka a i ʻole plasma, ka mea i hoʻohana nui ʻia e loiloi i nā pae o Estradiol. pono e hōʻoia ʻia e nā ʻano hana ʻē aʻe. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

HOOLAHA
ʻO Estradiol (E2) ka hormone koʻikoʻi a ikaika loa i ka estrogen. ʻO kona kaumaha molekala he 272.3 D. Ma ka laulā, no nā wahine hāpai ʻole, ua hūnā ʻia ʻo E2 e ka sheath and granular cell and luteal cell during follicular development.During pregnancy, Hoʻopili nui ʻia ka E2 e ka placenta, ʻoiai nā kāne ka mea i hana nui ʻia e nā testicles. Ma hope o ke komo ʻana o E2 i ke koko, 1% a 3% ʻaʻole e hoʻopaʻa i nā protein, 40% e hoʻopaʻa i ka sex hormone binding globulin (SHBG), a me nā mea ʻē aʻe. albumin, metabolize mai ke akepaʻa i loko o ka wai-soluble sulfates a gluconaldehyde esters, a excreted mai ka mimi.E2 mea he nui hormone hōʻailona no ka loiloi 'ana ovarian hana.

KUMU O KE HANA
Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka conjugate o BSA a me Estradiol ma ka ʻāpana hoʻāʻo a me ke kao anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka pahu marker e ka hōʻailona fluorescence anti E2 antibody a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana, E2 i ka laʻana e hui pū me ka fluorescence i hōʻailona ʻia i ka anti E2 antibody, a hana i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i ke kuhikuhi o absorbent pepa, i ka wa paʻakikī i hala i ka ho'āʻo 'āina, E hui pu me ka Estradiol ka hoailona fluorescent ma ka membrane.O ka noonoo ana o Estradiol mea ino correlation no ka fluorescence hoailona, ​​a me ka Hiki ke ʻike ʻia ka neʻe ʻana o Estradiol i ka laʻana e ka fluorescence immunoassay assay.

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：
.Kale ho'āʻo pakahi pahu pepa me ka desiccant 25T
.He haʻina 25T
.B hāʻina 1
.Hoʻokomo pūʻolo 1

MAKEMAKEIA NA MEA KALAU AOLE I HANAIA
Laʻana hōʻiliʻili ipu, manawa

OHI A ME KA WAIHANA
1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.
2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana. Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
.A'ole ka la'ana a pau i ka ho'ohehe'e hau.

HANA ANA
ʻO ke kaʻina hana hoʻāʻo o ka mea kani e ʻike i ka manual immunoanalyzer. Penei ke kaʻina hana ho'āʻo reagent
1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
3. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
4. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hoʻāʻo.
5.Add 30μL serum a plasma sample i A solution, a hui maikaʻi.
6.Add 20μL B solution i ka hui luna, a hui maikaʻi.
E waiho i ka hui no20minuke.
E hoʻohui i ka hui ʻana o 80μL e hoʻāʻo maikaʻi i ke kāleka.
Kaomi i ke pihi "hoʻāʻo maʻamau", ma hope o 10 mau minuke, e ʻike koke ka mea hana i ke kāleka hōʻike, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena hōʻike.
E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

HUA HOʻOʻO A ME KA HOʻOKAʻANA

.ʻO ka ʻikepili ma luna nei ka wā kuhikuhi i hoʻokumu ʻia no ka ʻike ʻike o kēia kit, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi manawa kuhikuhi no ke ʻano koʻikoʻi pili o ka heluna kanaka ma kēia māhele.
ʻOi aku ka kiʻekiʻe o ka Estradiol ma mua o ka pae kuhikuhi, a pono e kāpae ʻia nā hoʻololi physiological a i ʻole ke koʻikoʻi.
.Hoʻopili wale nā ​​hopena o kēia ʻano i ka pae kuhikuhi i hoʻokumu ʻia e kēia ʻano, a ʻaʻole hoʻohālikelike nā hopena me nā ʻano hana ʻē aʻe.
. Hiki i nā kumu ʻē aʻe ke hana hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA
1. ʻO ka pahu he 18 mahina mālama ola mai ka lā o ka hana ʻana. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. MAI HOOMAIKAI. Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a ua manaʻo ʻia ka hoʻohana hoʻokahi hoʻohana e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, humidity 40-90%) i loko o 60 mins me ka wikiwiki. e like me ka hiki.
Hoʻohana koke ʻia ka diluent 3.Sample ma hope o ka wehe ʻana.

NA ALA A ME KA MALAMA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
Mai hoʻohana i ka reagent i pau.
Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻokoʻa ʻole.
Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.Misoperation, excessive or liʻiliʻi hāpana hiki ke alakai i ka hopena deviations.

LHOOLAHA
.E like me kekahi ho'ā'o e ho'ohana ana i ka 'iole antibodies, hiki ke ho'opilikia 'ia e ke kanaka anti-mouse antibodies (HAMA) i ka specimen. Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau. Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
.ʻO kēia hopena ho'āʻo wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lawelawe ʻo ia wale nō ke kumu no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi maʻi me kāna mau hōʻailona.
.moʻolelo lapaʻau, hoʻokolohua ʻē aʻe, pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe.
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.
NA HIO HANA

RHOOLAHA
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana

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