ʻO ka pahu Diagnostic no ka huina Triiodothyronine(ka ho'āʻo immunochromatographic fluorescence)
No ka hoʻohana diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA
ʻO ka Diagnostic Kit for Total Triiodothyronine (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike quantitative o Total Triiodothyronine (TT3) i loko o ke kanaka serum a i ʻole plasma, ka mea i hoʻohana nui ʻia e loiloi i ka hana thyroid. pono e hōʻoia ʻia e nā ʻano hana ʻē aʻe. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

HOOLAHA
Triiodothyronine(T3) molekala kaumaha 651D. ʻO ia ke ʻano hana nui o ka hormone thyroid. Hoʻokaʻawale ʻia ka huina T3 (T3, TT3) i ka serum i nā ʻano paʻa a me nā ʻano manuahi. ʻO 99.5 % o TT3 e hoʻopaʻa ana i ka serum Thyroxine Binding Proteins (TBP), a me ka T3 manuahi (Free T3) helu no 0.2 a 0.4 %. Hoʻohana ʻia ʻo T4 a me T3 i ka mālama ʻana a me ka hoʻoponopono ʻana i ka hana metabolic o ke kino. ʻO ka Clinical TT3 kahi hōʻailona hilinaʻi no ka hōʻailona a me ka nānā pono o ka hyperthyroidism a me ka hypothyroidism.

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka conjugate o BSA a me T3 ma ka ʻāpana hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka pahu marker e ka hōʻailona fluorescence anti T3 antibody a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana, TT3 i ka laʻana e hui pū me ka fluorescence i hōʻailona ʻia i ka anti T3 antibody, a hana i ka hui ʻole. Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i ke kuhikuhi o ka absorbent pepa, i ka wā paʻakikī i hala i ka ho'āʻo 'āina, E hui pū ka mea hoailona fluorescent manuahi me T3 ma ka membrane. ʻO ka manaʻo o TT3 ka hoʻopili maikaʻi ʻole no ka hōʻailona fluorescence, a me ka Hiki ke ʻike ʻia ka neʻe ʻana o TT3 i ka laʻana e ka fluorescence immunoassay assay.

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：
.Kale ho'āʻo pakahi pahu pepa me ka desiccant 25T
.He haʻina 25T
.B hāʻina 1
.Hoʻokomo pūʻolo 1

MAKEMAKEIA NA MEA KALAU AOLE I HANAIA
Laʻana hōʻiliʻili ipu, manawa

OHI A ME KA WAIHANA
1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.
2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana. Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
3.All laʻana e pale i nā pōʻaiapuni freeze-thaw.

HANA ANA
ʻO ke kaʻina hana hoʻāʻo o ka mea kani e ʻike i ka manual immunoanalyzer. Penei ke kaʻina hana ho'āʻo reagent
1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
6.Add 30μL serum a plasma sample i A solution, a hui pono.
7.Add 20μL B solution i ka hui luna, a hui maikaʻi.
8. E waiho i ka hui no 20 mau minuke.
9.Add 80μL hui 'ia e laʻana maikaʻi o ke kāleka.
10. Kaomi i ke pihi "ho'āʻo maʻamau", ma hope o 10 mau minuke, e ʻike koke ka mea kani i ka kāleka hōʻike, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena hoʻāʻo.
11. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

NA WAIWAI I MANAOIA

TT3 laulā maʻamau:0.5-2.5ng/mL
Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hana i kona ʻano maʻamau e hōʻike ana i kona heluna maʻi.

HUA HOʻOʻO A ME KA HOʻOKAʻANA
.ʻO ka ʻikepili ma luna nei ka wā kuhikuhi i hoʻokumu ʻia no ka ʻike ʻike o kēia kit, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi manawa kuhikuhi no ke ʻano koʻikoʻi pili o ka heluna kanaka ma kēia māhele.
.ʻOi aku ka kiʻekiʻe o ka TT3 ma mua o ka pae kuhikuhi, a pono e kāpae ʻia nā hoʻololi physiological a i ʻole ke koʻikoʻi.
.Hoʻopili wale nā ​​hopena o kēia ʻano i ka pae kuhikuhi i hoʻokumu ʻia e kēia ʻano, a ʻaʻole hoʻohālikelike nā hopena me nā ʻano hana ʻē aʻe.
. Hiki i nā kumu ʻē aʻe ke hana hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA
1. ʻO ka pahu he 18 mahina mālama ola mai ka lā o ka hana ʻana. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. MAI HOOMAIKAI. Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a ua manaʻo ʻia ka hoʻohana hoʻokahi hoʻohana e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, humidity 40-90%) i loko o 60 mins me ka wikiwiki. e like me ka hiki.
Hoʻohana koke ʻia ka diluent 3.Sample ma hope o ka wehe ʻana.

NA ALA A ME KA MALAMA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
Mai hoʻohana i ka reagent i pau.
Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻokoʻa ʻole.
Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.Misoperation, excessive or liʻiliʻi hāpana hiki ke alakai i ka hopena deviations.

LHOOLAHA
.E like me kekahi ho'ā'o e ho'ohana ana i ka 'iole antibodies, hiki ke ho'opilikia 'ia e ke kanaka anti-mouse antibodies (HAMA) i ka specimen. Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau. Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi i ka mālama ʻana i ka maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe. .
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NA HIO HANA

RHOOLAHA
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana

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