Kit Diagnostic noLuteinizing Hormoneka hōʻike immunochromatographic fluorescence)
No ka hoʻohana diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA
Diagnostic Kit for Luteinizing Hormone (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike nui ʻana o Luteinizing Hormone (LH) i loko o ke kanaka serum a i ʻole plasma, ka mea i hoʻohana nui ʻia i ka loiloi o ka hana endocrine pituitary. nā ʻano hana ʻē aʻe. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

HOOLAHA

ʻO ka hormone luteinizing (LH) he glycoprotein me ke kaumaha molekala ma kahi o 30,000 Dalton, i hana ʻia e ka pituitary anterior. ʻO ka hoʻopaʻa ʻana o ka LH pili pili i ka ovulation o nā ovaries, a ʻo ke kiʻekiʻe o LH i wānana ʻia he 24 a 36 mau hola o ka ovulation. No laila, hiki ke nānā ʻia ka waiwai kiʻekiʻe o LH i ka wā o ka menstrual cycle e hoʻoholo ai i ka manawa kūpono o ka hāpai ʻana. Hiki i ka hana endocrine maʻamau i loko o ka pituitary gland ke hana i ka LH huna irregularity. Hiki ke hoʻohana ʻia ka ʻike o LH e loiloi i ka hana endocrine pituitary. Hoʻokumu ʻia ka Diagnostic Kit ma ka immunochromatography a hiki ke hāʻawi i kahi hopena i loko o 15 mau minuke.

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka anti LH antibody ma ka ʻāina hoʻāʻo a me ke kao anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia i ka anti LH antibody a me ka rabbit IgG ma mua. I ka ho'āʻo ʻana i ka laʻana maikaʻi, hui pū ka LH antigen i ka laʻana me ka fluorescence i kapa ʻia i ka anti LH antibody, a hana i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, ke kahe paʻakikī i ke kuhikuhi o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū ʻia me ka anti LH coating antibody, hana hou i ka paʻakikī. Hoʻopili maikaʻi ʻia ka pae LH me ka hōʻailona fluorescence, a hiki ke ʻike ʻia ka neʻe ʻana o LH i ka hāpana e ka fluorescence immunoassay assay.

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：

.Kale ho'āʻo pakahi pahu pepa me ka desiccant 25T
.Laʻana diluents
.Hoʻokomo pūʻolo

MAKEMAKEIA NA MEA KALAU AOLE I HANAIA
Laʻana hōʻiliʻili ipu, manawa

OHI A ME KA WAIHANA
1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.
2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana. Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
3.All laʻana e pale i nā pōʻaiapuni freeze-thaw.

HANA ANA

ʻO ke kaʻina hana hoʻāʻo o ka mea kani e ʻike i ka manual immunoanalyzer. Penei ke kaʻina hana ho'āʻo reagent
1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
6.Add 20μL serum a plasma hāpana e laʻana diluent, a hui pono..
7.Add 80μL hāpana hāpana no ka laʻana maikaʻi o ke kāleka.
8. E kaomi i ke pihi "ho'āʻo maʻamau", ma hope o nā minuke 15, eʻike pono ka mea hana i ke kāleka ho'āʻo, hiki iā ia ke heluhelu i nā hopena mai ka hōʻikeʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena ho'āʻo.
9. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

HUA HOʻOʻO A ME KA HOʻOKAʻANA

.ʻO ka ʻikepili ma luna nei ka wā kuhikuhi i hoʻokumu ʻia no ka ʻike ʻike o kēia kit, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi manawa kuhikuhi no ke ʻano koʻikoʻi pili o ka heluna kanaka ma kēia māhele.
ʻOi aku ka kiʻekiʻe o ka LH ma mua o ka pae kuhikuhi, a pono e kāpae ʻia nā loli physiological a i ʻole ke koʻikoʻi.
.Hoʻopili wale nā ​​hopena o kēia ʻano i ka pae kuhikuhi i hoʻokumu ʻia e kēia ʻano, a ʻaʻole hoʻohālikelike nā hopena me nā ʻano hana ʻē aʻe.
. Hiki i nā kumu ʻē aʻe ke hana hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA
1. ʻO ka pahu he 18 mahina mālama ola mai ka lā o ka hana ʻana. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. MAI HOOMAIKAI. Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a ua manaʻo ʻia ka hoʻohana hoʻokahi hoʻohana e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, humidity 40-90%) i loko o 60 mins me ka wikiwiki. e like me ka hiki.
Hoʻohana koke ʻia ka diluent 3.Sample ma hope o ka wehe ʻana.

NA ALA A ME KA MALAMA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
Mai hoʻohana i ka reagent i pau.
. ʻAʻole hoʻololi i nā reagents ma waena o nā pahu me nā ʻokoʻa ʻokoʻa No..
Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.Misoperation, excessive or liʻiliʻi hāpana hiki ke alakai i ka hopena deviations.

LHOOLAHA
.E like me kekahi ho'ā'o e ho'ohana ana i ka 'iole antibodies, hiki ke ho'opilikia 'ia e ke kanaka anti-mouse antibodies (HAMA) i ka specimen. Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau. Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi i ka mālama ʻana i ka maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe. .
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NA HIO HANA

HOOLAHA
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana

Xiamen Wiz Biotech CO.,LTD
Wahi: 3-4 Papa, NO.16 Hale, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Kina
Tel:+86-592-6808278
Fax:+86-592-6808279