Kit Diagnostic noCalprotectin(Fluorescence Immunochromatographic Assay)
No ka hoʻohana diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA
Kit Diagnostic noCalprotectin(Cal) ua kūpono no ka quantitative hoʻoholo 'ana o kanaka faeces Cal ma ka fluorescence immunochromatographic assay, he mea koʻikoʻi accessory diagnostic waiwai no ka inflammatory bowel maʻi.All maikaʻi hāpana pono e hōʻoiaʻiʻo e kekahi methodologies. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

HOOLAHA
He heterodimer ʻo Cal, i haku ʻia me MRP 8 a me MRP 14^[1]. Aia ia i loko o ka neutrophils cytoplasm a hōʻike ʻia ma nā membrane cell mononuclear. ʻO Cal he mau proteins acute phase, loaʻa iā ia kahi pae paʻa maikaʻi ma kahi o hoʻokahi pule i loko o nā faeces kanaka, ua hoʻoholo ʻia he hōʻailona maʻi ʻōpū.^[2-3]. ʻO ka pahu kahi hoʻāʻo maʻalahi, ʻike nui e ʻike iā Cal i loko o nā faeces kanaka, he kiʻekiʻe ka ʻike ʻike a me ka kikoʻī ikaika. ʻO ka hoʻāʻo e pili ana i ka kikoʻī kiʻekiʻe o ka ʻenehana pane ʻana i nā antibodies ʻelua a me ka fluorescence immunochromatographic assay analysis technics, hiki ke hāʻawi i kahi hopena i loko o 15 mau minuke.

KUMU O KE HANA
He anti-Cal coating antibody ma ka ʻāpana hoʻāʻo, i hoʻopaʻa ʻia i ka chromatography membrane ma mua. Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia he anti-Cal antibody ma mua. I ka ho'āʻo ʻana i ka laʻana maikaʻi, hiki ke hoʻohui ʻia ka Cal i ka laʻana me ka fluorescence i kapa ʻia i ka anti-Cal antibody, a hana i ka hui kūlohelohe. I ka ʻae ʻia ʻana o ka hui ʻana e neʻe ma ka ʻaoʻao hoʻāʻo, hopu ʻia ka paʻakikī Cal conjugate e ka anti-Cal coating antibody ma ka membrane a lilo i paʻakikī. Hoʻopili maikaʻi ʻia ka ikaika fluorescence me ka ʻike Cal. Hiki ke ʻike ʻia ka Cal ma ka laʻana e ka fluorescence immunoassay analyzer.

NA REAGENA a me na lako i hoolakoia

25T pūʻolo mea：
Kāleka hoʻāʻo i hoʻokahi pahu i hoʻopaʻa ʻia me kahi desiccant 25T
Nā mea hoʻoheheʻe laʻana 25T
Hoʻokomo pūʻolo 1

MAKEMAKEIA NA MEA KALAU AOLE I HANAIA
Laʻana hōʻiliʻili ipu, manawa

OHI A ME KA WAIHANA
1.E hoʻohana i kahi pahu maʻemaʻe hoʻohana e hōʻiliʻili i ka hāpana faeces hou, a hoʻāʻo koke ʻia. Inā ʻaʻole hiki ke hoʻāʻo koke ʻia, e ʻoluʻolu e mālama ʻia ma 2-8°C no 3 mau lā a i ʻole -15°C no 6 mahina.
2. E lawe i ka laʻau laʻau, hoʻokomo ʻia i loko o ka laʻana faeces, e hana hou i ka hana 3 manawa, e lawe i nā ʻāpana like ʻole o ka laʻana faeces i kēlā me kēia manawa, a laila e hoʻihoʻi i ka lāʻau laʻau, wili a hoʻoluliluli maikaʻi, A i ʻole ka hoʻohana ʻana i ka lāʻau laʻau i koho ʻia. e pili ana i 50mg faeces laʻana, a hoʻokomo i loko o ka faeces hāpana paipu i loaʻa ka laʻana dilution, a wili paʻa.
3. E hoʻohana i ka laʻana pipette disposable e lawe i ka laʻana faeces mai ka maʻi maʻi maʻi, a laila e hoʻohui i 3 kulu (e pili ana i 100µL) i ka paipu hoʻoheheʻe fecal a lulu maikaʻi.

Nā memo:
1. Avoid i nā pōʻai hau hau.
2.Thaw laʻana i ka lumi wela ma mua o ka hoʻohana.

HANA ANA

E ʻoluʻolu e heluhelu i ka manual operation instrument a me ka hoʻokomo pūʻolo ma mua o ka hoʻāʻo ʻana.
1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
6. Wehe i ka pāpale mai ka hāpana paipu a hoʻolei i nā kulu mua ʻelua i hoʻoheheʻe ʻia, hoʻohui i 3 kulu (e pili ana i 100uL) ʻaʻohe pahu i hoʻoheheʻe ʻia i ka hāpana verticaly a me ka mālie i loko o ka luawai o ke kāleka me ka dispette i hāʻawi ʻia.
7. E kaomi i ke pihi "ho'āʻo maʻamau", ma hope o 15 mau minuke, e ʻike koke ka mea hana i ka kāleka hōʻike, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena hōʻike.
8. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

NA WAIWAI I MANAOIA
Cali <60μg/g
Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hana i kona ʻano maʻamau e hōʻike ana i kona heluna maʻi.

HUA HOʻOʻO A ME KA HOʻOKAʻANA
1. ʻO ka Cal i ka laʻana heʻoi aku ka nui ma mua o 60μg / g, a pono e hoʻoholo i ka hoʻololiʻana o ke kūlana physiological. He ʻano ʻē nā hopena a pono e ʻike ʻia me nā hōʻailona lapaʻau.
2. Hoʻopili wale nā ​​hopena o kēia ʻano i nā pae kuhikuhi i hoʻokumu ʻia ma kēia ʻano, a ʻaʻohe hoʻohālikelike pololei me nā ʻano hana ʻē aʻe.
3. Hiki i nā mea ʻē aʻe ke hana i nā hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

HOOLAHA A ME KA PALAPALA
1. ʻO ka pahu he 18 mahina mālama ola mai ka lā o ka hana ʻana. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. MAI HOOMAIKAI. Mai hoʻohana ma mua o ka lā pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a ua manaʻo ʻia ka hoʻohana hoʻokahi hoʻohana e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, humidity 40-90%) i loko o 60 mins me ka wikiwiki. e like me ka hiki.
Hoʻohana koke ʻia ka diluent 3.Sample ma hope o ka wehe ʻana.

NA ALA A ME KA MALAMA
. Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.
.E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
.E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
Mai hoʻohana i ka reagent i pau.
Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻāpana ʻokoʻa No..
Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
.Misoperation, excessive or liʻiliʻi hāpana hiki ke alakai i ka hopena deviations.

HOOLAHA
.E like me kekahi ho'ā'o e ho'ohana ana i ka 'iole antibodies, hiki ke ho'opilikia 'ia e ke kanaka anti-mouse antibodies (HAMA) i ka specimen. Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau. Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi i ka mālama ʻana i ka maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe. .
.Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua fecal. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NA HIO HANA

HOOLAHA
1.Li,G.&Y.L.Li.Ka pilina ma waena o ka calcium a me ka ma'i ma'i [J].The Journal of Practical Medicine,2007,23(15)
2.Han,W.,Xu,JM,et al.Ke aʻo hoʻomaʻamaʻa o ka calamine a me ka lactoferrin i nā maʻi me ka ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al.The study on the relationship between fecal calamine and inflammatory bowel disease[J]. ʻenehana ʻepekema a me ka ʻenekinia,2010-03,10(8)

Ki i nā hōʻailona i hoʻohana ʻia:

	In Vitro Diagnostic Lapaʻau Lapaʻau
	Mea hana
	E mālama i ka 2-30 ℃
	Ka lā pau
	Mai hoohana hou
	ALOHA
	E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana

Xiamen Wiz Biotech CO.,LTD
Wahi: 3-4 Papa, NO.16 Hale, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Kina
Tel:+86-592-6808278
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