Kiko Kikokiko（Gula Koloidal）no Calprotectin
No ka hoʻohana diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA
ʻO ka Diagnostic Kit no Calprotectin(cal) he colloidal gula immunochromatographic assay no ka hoʻoholo semiquantitative o ka cal mai nā faeces kanaka, nona ka waiwai diagnostic accessory koʻikoʻi no ka maʻi ʻeha o ka ʻōpū. ʻO kēia ho'āʻo he mea hoʻoponopono kānana. Pono e hōʻoia ʻia ka laʻana maikaʻi a pau e nā ʻano hana ʻē aʻe. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō. I kēia manawa, hoʻohana ʻia kēia hōʻike no IVD, ʻaʻole pono nā mea hana hou.

HOOLAHA
He heterodimer ka Cal, i haku ʻia me MRP 8 a me MRP 14. Aia i loko o ka neutrophils cytoplasm a hōʻike ʻia ma nā membrane cell mononuclear. ʻO Cal he mau proteins acute phase, loaʻa iā ia kahi pae paʻa e pili ana i hoʻokahi pule i loko o nā faeces kanaka, ua hoʻoholo ʻia he hōʻailona maʻi ʻeha. He ho'āʻo semiqualitative maʻalahi ke kit e ʻike i ka cal i loko o nā faeces kanaka, he kiʻekiʻe ka ʻike ʻike a me ka kikoʻī ikaika. ʻO ka hoʻāʻo e pili ana i ke ʻano kiʻekiʻe specificit double antibodies sandwich reaction principle a me ke gula immunochromatographic assay analysis technics, hiki iā ia ke hāʻawi i kahi hopena i loko o 15 mau minuke.

KUMU O KE HANA
Loaʻa i ke kaula ka uhi ʻana i ka anti cal McAb ma ka ʻāina hoʻāʻo a me ka anti-rabbit IgG antibody ma ka ʻāina hoʻomalu, i hoʻopaʻa ʻia i ka chromatography membrane ma mua. Ua uhi ʻia ka Lable pad e ke gula colloidal i kapa ʻia ʻo anti cal McAb a me ke gula colloidal i kapa ʻia ʻo rabbit IgG antibody ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi, ua hui ʻia ka cal i loko o ka laʻana me ke gula colloidal i kapa ʻia ʻo anti cal McAb, a hana i ka paʻakikī immune, no ka mea e ʻae ʻia e neʻe ma ka ʻāpana hoʻāʻo, hopu ʻia ka paʻakikī cal conjugate e ka anti cal coating McAb ma ka membrane a me ke ʻano. ʻO ka paʻakikī "anti cal coating McAb-cal-colloidal gula i kapa ʻia he anti cal McAb", ua ʻike ʻia kahi hui hoʻāʻo kala ma ka ʻāina hoʻāʻo. Hoʻopili maikaʻi ʻia ka ikaika kala me ka maʻiʻo cal. ʻAʻole hoʻopuka kahi laʻana maikaʻi ʻole i kahi hui hoʻāʻo ma muli o ka loaʻa ʻole o ka colloidal gula conjugate cal complex. ʻAʻohe mea i loaʻa i ka laʻana a ʻaʻole paha, aia kahi ʻulaʻula e ʻike ʻia ma ka ʻāina kuhikuhi a me ka ʻāina hoʻomalu maikaʻi, i manaʻo ʻia ʻo ia nā kūlana ʻoihana kūloko.

NA REAGENA a me na lako i hoolakoia
25T pūʻolo mea：
. Kāleka hoʻāʻo pakahi i hoʻopaʻa ʻia me kahi desiccant
.Sample diluents: nā mea hoʻohui he 20mM pH7.4PBS
.Dispette
.Hoʻokomo pūʻolo

MAKEMAKEIA NA MEA KALAU AOLE I HANAIA

ipu hōʻiliʻili laʻana, manawa

OHI A ME KA WAIHANA
E hoʻohana i kahi ipu maʻemaʻe hoʻopau e hōʻiliʻili i ka laʻana faeces hou, a hoʻāʻo koke ʻia. Inā ʻaʻole hiki ke hoʻāʻo koke ʻia, e ʻoluʻolu e mālama ʻia ma 2-8 ° C no 12 hola a i ʻole -15 ° C no 4 mau mahina.

HANA ANA
1. Wehe i ka lāʻau laʻau, hoʻokomo ʻia i loko o ka laʻana faeces, a laila hoʻihoʻi i ka lāʻau laʻau, wili a hoʻoluliluli maikaʻi, e hana hou i ka hana 3 manawa. A i ʻole ka hoʻohana ʻana i ka laʻana lāʻau stickpicked ma kahi o 50mg faeces laʻana, a hoʻokomo i loko o ka faeces sample tube i loaʻa ka laʻana dilution, a wili paʻa.
2. E hoʻohana i ka laʻana pipette disposable e lawe i ka laʻana faeces thinner mai ka mea maʻi maʻi maʻi, a laila e hoʻohui i 3 kulu (e pili ana i 100uL) i ka paipu hoʻoheheʻe fecal a lulu maikaʻi, waiho ʻia.
3. E lawe i ke kāleka hoʻāʻo mai ka ʻeke pepa, e kau ma luna o ka pākaukau pae a kaha.
4.Remove ka pāpale mai ka hāpana paipu a hoolei i ka mua elua kulu diluted hāpana, hoʻohui 3 kulu (e pili ana i 100uL) ole bubble diluted hāpana verticaly a me ka lohi i ka laʻana luawai o ke kāleka me ka hoʻolako dispette, hoʻomaka manawa.
5. Pono e heluhelu ʻia ka hopena i loko o nā minuke 10-15, a ʻaʻole kūpono ia ma hope o 15 mau minuke.

HUA HOʻOʻO A ME KA HOʻOKAʻANA

HOOLAHA A ME KA PALAPALA
ʻO ka pahu he 24 mahina mālama ola mai ka lā i hana ʻia ai. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a mākaukau ʻoe e hana i kahi hoʻokolohua.
OLELO HOOLAHA A ME KA MALAMA
1. Pono e hoʻopaʻa ʻia ka kit a pale ʻia mai ka wai¹.
2. Mai hoʻohana i ka laʻana i waiho lōʻihi loa a i ʻole ka hoʻoheheʻe hou ʻana a me ka hoʻoheheʻe ʻana e hoʻāʻo
3. Fecal laʻana mea excessive a mānoanoa hiki ke hana i ka diluted Eia kekahi laʻana ho'āʻo kāleka, eʻoluʻolu centrifuge ka diluted hāpana a lawe i ka supernatant no ka hoao ana.
4.Misoperation, excessive a liʻiliʻi paha laʻana hiki ke alakai i ka hopena deviations.

HOOLAHA
1. ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia ka hoʻokele ʻana o nā maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā mea ʻē aʻe. ʻike².
2. Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua fecal. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.