ʻO ka pahu diagnostic no ka Procalcitonin

(ka ho'āʻo immunochromatographic fluorescence)

No ka hoʻohana diagnostic in vitro wale nō

E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi. ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

KA MANAO ANA

ʻO ka Diagnostic Kit for Procalcitonin (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike quantitative o Procalcitonin (PCT) i loko o ke kanaka serum a i ʻole plasma, ua hoʻohana ʻia no ka hōʻailona kōkua o ka maʻi bacterial a me ka sepsis. Pono e hōʻoia ʻia ka laʻana maikaʻi a pau e nā ʻano hana ʻē aʻe. Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

HOOLAHA

Hoʻokumu ʻia ka Procalcitonin me 116 amino acids a ʻo kona kaumaha molekala he 12.7KD. Hōʻike ʻia ka PCT e nā pūnaewele neuroendocrine a wāwahi ʻia e nā enzymes i loko o ka calcitonin (immature), carboxy-terminating peptide, a me amino terminating peptide. He wahi liʻiliʻi wale nō ka PCT i loko o ke koko o ka poʻe olakino, hiki ke hoʻonui nui ʻia ma hope o ka maʻi bacteria. Ke loaʻa ka sepsis i loko o ke kino, hiki i ka hapa nui o nā ʻiʻo ke hōʻike i ka PCT, no laila hiki ke hoʻohana ʻia ka PCT ma ke ʻano he hōʻailona prognostic o sepsis. No kekahi poʻe maʻi me ka maʻi ʻeha, hiki ke hoʻohana ʻia ka PCT ma ke ʻano he hōʻailona o ke koho antibiotic a me ka hoʻoholo kūpono.

KUMU O KE HANA

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka anti-PCT antibody ma ka ʻāina hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka Lable pad e ka fluorescence i kapa ʻia i ka anti-PCT antibody a me ka rabbit IgG ma mua. I ka ho'āʻo ʻana i ka laʻana maikaʻi, hui pū ka PCT antigen i ka laʻana me ka fluorescence i kapa ʻia i ka anti-PCT antibody, a hoʻokumu i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, ke kahe paʻakikī i ke kuhikuhi o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū me ka anti-PCT coating antibody, hana hou i ka paʻakikī. Hoʻopili maikaʻi ʻia ka pae PCT me ka hōʻailona fluorescence, a hiki ke ʻike ʻia ka neʻe ʻana o ka PCT i ka laʻana e ka fluorescence immunoassay assay.