KUMU

Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me Rotavirus Group A antigen ma ka ʻāina hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāina hoʻomalu. Hoʻopili ʻia ka lable pad e ka fluorescence i kapa ʻia he anti Rotavirus Group A a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi, hui pū ka RV i ka laʻana me ka fluorescence i kapa ʻia ʻo Anti-Rotavirus Group A, a hana i ka hui kūlohelohe. Ma lalo o ka hana o ka immunochromatography, kahe ka paʻakikī i ke kuhikuhi o ka pepa absorbent. I ka hala ʻana o ka paʻakikī i ka ʻāina hoʻāʻo, ua hui pū ʻia me ka anti-Rotavirus Group A coating antibody, e hana i kahi paʻakikī hou. Inā maikaʻi ʻole, ʻaʻohe Rotavirus Group A antigen i ka hāpana, no laila ʻaʻole hiki ke hoʻokumu ʻia nā paʻakikī immune, ʻaʻohe laina ʻulaʻula ma ka wahi ʻike (T). ʻAʻohe o ka hui A rotavirus i loko o ka specimen, ka latex-labeled mouse IgG i chromatographed i ka maikaʻi wahi (C) a hopu ʻia e kao anti-mouse IgG antibody. E ʻike ʻia kahi laina ʻulaʻula ma ka wahi hoʻomalu maikaʻi (C). ʻO ka laina ʻulaʻula ka maʻamau i hōʻike ʻia ma ka ʻāpana hoʻomalu maikaʻi (C) no ka hoʻoholo ʻana inā lawa nā laʻana a inā he maʻamau ke kaʻina chromatography. Hoʻohana ʻia ia ma ke ʻano he maʻamau mana kūloko no nā reagents.

Kaʻina hana hoʻāʻo:

Pono e hōʻiliʻiliʻia nā maʻi maʻi hōʻailona 1. Wahi a nā hōʻike, ʻo ka excretion kiʻekiʻe loa o ka rotavirus i loko o nā faeces o nā mea maʻi me ka gastroenteritis e kū mai 3-5 mau lā ma hope o ka hoʻomaka ʻana o ka maʻi a me 3-13 mau lā ma hope o ka hoʻomaka ʻana o nā hōʻailona. Inā hōʻiliʻili ʻia ka hāpana ma hope o ka maʻi ʻana, ʻaʻole lawa paha ka nui o nā antigens e loaʻa ai ka hopena maikaʻi.

2. Pono e hōʻiliʻili ʻia nā laʻana i loko o kahi pahu maʻemaʻe, maloʻo, wai wai ʻaʻole i loaʻa nā detergents a me nā preservatives.

3. No nā maʻi maʻi ʻole, ʻaʻole pono e emi iho nā ʻano faeces i hōʻiliʻili ʻia ma mua o 1-2 grams. No nā poʻe maʻi me ka diarrhea, inā he wai ka feces, e ʻoluʻolu e hōʻiliʻili ma kahi o 1-2 ml o ka wai faeces. Inā nui ke koko a me ka mucus o ka feces, e ʻoluʻolu e ʻohi hou i ka hāpana.

4. Manaʻo ʻia e hoʻāʻo i nā laʻana ma hope koke o ka hōʻiliʻili ʻana, i ʻole e hoʻouna ʻia i ka hale hana i loko o 6 mau hola a mālama ʻia ma 2-8 ° C. Inā ʻaʻole i hoʻāʻo ʻia nā laʻana i loko o 72 mau hola, pono e mālama ʻia ma ka mahana ma lalo o -15 ° C.

5.E hoʻohana i nā feces hou no ka hoʻāʻo ʻana, a me nā laʻana faeces i hui pū ʻia me ka diluent a i ʻole ka wai i hoʻoheheʻe ʻia