KA'IDA

Tsiri yana da antigen mai rufi na MP-Ag akan yankin gwaji da goat anti linzamin kwamfuta IgG antibody akan yankin sarrafawa, wanda aka ɗaure zuwa membrane chromatography a gaba. Label pad an lullube shi da zinare mai alamar linzamin kwamfuta- anti ɗan adam IgM McAb a gaba. Lokacin gwada ingantaccen samfurin, MP-IgM a cikin samfurin yana haɗuwa tare da zinare mai launi mai lakabin linzamin kwamfuta-anti ɗan adam IgM McAb, kuma yana samar da hadaddun rigakafi. A karkashin aikin immunochromatography, hadaddun da samfurin ciki na nitrocellulose membrane kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, shi a hade tare da MP-Ag shafi antigen, forming "MP-Ag shafi antigen-MP. -IgM-colloidal zinare mai lakabin linzamin kwamfuta-anti ɗan adam IgM McAb” hadaddun, ƙungiyar gwaji mai launi ta bayyana akan yankin gwaji. Samfura mara kyau baya samar da band ɗin gwaji saboda ƙarancin hadaddun rigakafi. Komai MP-IgM ya kasance a cikin samfur ko a'a, akwai jajayen ratsin ja ya bayyana akan yankin kula da inganci, wanda ake ɗaukarsa azaman ingantattun ka'idojin kasuwanci na ciki.

Tsarin Gwaji:

Hanyar gwajin WIZ-A101 duba umarnin mai nazarin rigakafi mai ɗaukar nauyi. Hanyar gwajin gani kamar haka:

1. Ajiye duk reagents da samfurori zuwa zafin jiki.
2. Cire katin gwajin daga jakar jakar, sanya shi a kan teburin matakin kuma yi alama.
3. Add 10μL serum ko plasma sample ko 20μL dukan jini samfurin zuwa samfurin rijiyar katin tare da bayar dispette, sa'an nan ƙara 100μL (game da 2-3 drop) samfurin diluent, fara lokaci.
4. Jira mafi ƙarancin mintuna 10-15 kuma karanta sakamakon, sakamakon ba shi da inganci bayan mintuna 15.