KA'IDA

Membran na'urar gwajin an lullube shi da rukunin A da adenovirus antigen akan yankin gwajin da goat anti zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar fluorescence mai lakabin anti Group A da adenovirus da zomo IgG a gaba. Lokacin gwada samfur mai inganci don Rukunin A da adenovirus, Rukunin A da adenovirus a cikin samfurin suna haɗuwa tare da walƙiya mai suna anti Rotavirus Group A da adenovirus, kuma suna samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda. Lokacin da hadaddun ya wuce yankin gwajin, haɗe shi da rukunin anti-Rotavirus A da maganin rigakafi na adenovirus, ya haifar da sabon hadaddun. Idan ba shi da kyau, babu Rotavirus Group A da adenovirus antigen a cikin samfurin, don haka ba za a iya samar da hadaddun rigakafi ba, ba za a sami jan layi a wurin ganowa (T). Ko da ko rukunin A rotavirus da adenovirus suna nan a cikin samfurin, linzamin kwamfuta mai lakabin latex IgG yana chromatographed zuwa yankin kula da inganci (C) kuma an kama shi ta goat anti-mouse IgG antibody. Jajayen layi zai bayyana a yankin kula da inganci (C). Layin ja shine ma'auni yana bayyana a cikin yankin kula da inganci (C) don yin hukunci ko akwai isassun samfurori kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani dashi azaman ma'aunin kulawa na ciki don reagents.

Tsarin Gwaji:

1. Bude hular bututun tarin samfurin. Kada a zubar da maganin a cikin kwalban.
2. Cire sandar samfurin, a saka a cikin samfurin najasa (ko amfani da sandar samfurin don ɗaukar kimanin 50mg na najasa), sannan a mayar da sandar samfurin baya, murƙushewa da girgiza sosai, maimaita aikin sau 3. Ɗauki wani ɓangare na samfurin najasar kowane lokaci. Bayan an yi samfurin, sanya sandar samfurin a cikin bututun tattara najasa mai ɗauke da sinadarin najasa, sannan a dunƙule digon ɗin sosai. Idan najasar majinyacin da ke da gudawa ta fi sirara, ana iya amfani da bambaro da za a iya zubarwa don yin samfur. Yin amfani da samfurin pipette da za a iya zubar da shi, ɗauki samfurin najasa mafi ƙanƙanta daga mai ciwon zawo, sannan ƙara digo 3 (kimanin 100uL) zuwa bututun samfurin najasar.
3. Girgiza samfurin da kyau kuma cire hular a kan tip ɗin digo sannan a ajiye a gefe.
4. Lokacin da aka adana a ƙananan zafin jiki, ya kamata a mayar da kit ɗin zuwa zafin jiki kafin amfani. Fitar da katin gwajin daga jakar jakar, sanya shi a kan teburin matakin kuma yi alama.
5. Cire hula daga bututun samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 3 saukad (kimanin 100uL) babu kumfa diluted samfurin a tsaye kuma a hankali a cikin samfurin rijiyar katin tare da bayar dispette, fara lokaci.
6. Ya kamata a karanta sakamakon a cikin minti 10-15, kuma ba shi da inganci bayan minti 15.