KA'IDA

Membran na'urar gwajin an lullube shi da microalbumin antigen akan yankin gwajin da goat anti zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin microalbumin da zomo IgG a gaba. Idan babu albumin a cikin fitsari, colloidal zinariya-labeled anti-Alb-labeled monoclonal antibody a kan colloidal zinariya takarda zai gudu a kan membrane tare da fitsari zuwa ganewa line, da kuma hade da Alb-rufi antigen tare da bayyane. layi. Kuma launi na layi ya fi duhu fiye da launi na layi a cikin yankin sarrafawa (C), wannan mummunan sakamako ne. Idan fitsarin ya ƙunshi albumin, za su yi gogayya da antigen mai rufin Alb akan membrane don ɗaure ƙayyadaddun wuraren rigakafin a kan tambarin anti-Alb-labeled monoclonal antibody. Yayin da adadin albumin a cikin fitsari ya karu, gwaji

Launi na layi zai zama haske da haske. Ana iya gano abun ciki na albumin a cikin fitsari Semi-quantitatively ta hanyar kwatanta wurin gano (T) tare da yankin sarrafawa (C). Wurin kula da ingancin (C) da yankin tunani (R) akan kit koyaushe zasu bayyana yayin gwajin, kuma basu da alaƙa da kasancewar albumin na fitsari. Ana iya amfani da yankin sarrafawa (C) da layin tunani (R) azaman maƙasudin kula da ingancin ciki don kit.

Tsarin Gwaji:

Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji. Narke samfurori zuwa zafin jiki kafin amfani.

1.Dauki katin gwaji daga jakar foil. Sanya shi a kwance a kwance kuma a yi alama.

2. Ɗauki samfurin fitsari tare da pipette mai zubarwa, jefar da farkon sau biyu na samfurin fitsari. Ƙara digo 3 (kimanin 100uL) na fitsari mara kumfa zuwa tsakiyar ramin samfurin katin gwajin a tsaye kuma fara lokaci.

3. Karanta sakamakon a cikin minti 10-15. Ba shi da inganci idan fiye da mintuna 15.