KA'IDA

Ana lulluɓe da membrane na na'urar gwajin da anti EV71 antibody akan yankin gwajin da goat anti zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin anti EV71 antibody da zomo IgG a gaba. Lokacin gwada ingantaccen samfurin, EV71 antigen a cikin samfurin yana haɗuwa tare da walƙiya mai lakabin anti EV71 antibody, kuma ya samar da cakuda rigakafi. A karkashin aiki na chromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, shi hade da anti EV71 shafi antibody, Forms sabon hadaddun.

Idan ba shi da kyau, samfurin ba ya ƙunshi enterovirus 71 IgM antibody, don haka ba za a iya samar da hadaddun rigakafi ba. Ba za a sami jan layi a wurin ganowa ba (T). Ko da ko Enterovirus 71 IgM antibody ya wanzu a cikin samfurin ko a'a, ragowar colloidal zinare mai lakabin linzamin kwamfuta anti-human IgM monoclonal antibody da goat anti-mouse IgG antibody mai rufi a cikin ingancin kula da yankin (C). Sa'an nan agglutinates suna haɓaka launi a cikin yankin kula da inganci, kuma layin ja zai bayyana a cikin (C). Layin ja shine ma'auni yana bayyana a cikin yankin kula da inganci (C) don yin hukunci ko akwai isassun samfurori kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani dashi azaman ma'aunin kulawa na ciki don reagents.

Tsarin Gwaji:

1. Samfuran da aka gwada na iya zama jini gaba ɗaya, gami da jini mai jijiya ko jini na gefe. Ba za a iya adana cikakken jini bayan an tattara shi ba. Ya kamata a yi amfani da ni ba da daɗewa ba bayan tattarawa.

2.Serum samfurori ana tattara su daidai da daidaitattun dabaru. Maganin rashin kunna zafi ba zai iya amfani da shi ba. Ba a ba da shawarar yin amfani da lipemic, turbid ko gurɓataccen magani ba. Bambance-bambancen kwayoyin halitta a cikin jini. Kuma hazo zai shafi sakamakon gwajin, irin waɗannan samfuran yakamata a sanya su a tsakiya ko tace kafin amfani.

3. Samfuran da aka gwada na iya zama heparin, Sodium citrate ko EDTA anticoagulant plasma.

4.According ga daidaitattun dabaru tattara samfurin. Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 3 kuma ana kiyaye ku a ƙasa -15 ° C na watanni 3.

5.All samfurin guje wa daskare-narke hawan keke.