KA'IDA

An lulluɓe jikin na'urar gwajin da FOB antibody akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin anti FOB antibody da zomo IgG a gaba. Lokacin gwada ingantaccen samfurin, FOB antigen a cikin samfurin yana haɗuwa tare da mai walƙiya mai lakabin anti FOB antibody, kuma ya samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda. Lokacin da hadaddun ya wuce yankin gwajin, haɗe shi da anti-FOB shafi antibody, samar da sabon hadaddun. Idan ba shi da kyau, samfurin ba ya ƙunshi haemoglobin na mutum ko abun ciki yana da ƙasa, ba za a iya samar da isassun ƙwayoyin rigakafi ba. Ba za a sami jan layi a wurin ganowa ba (T). Layin ja shine ma'auni yana bayyana a cikin yankin kula da inganci (C) don yin hukunci ko akwai isassun samfurori kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani dashi azaman ma'aunin kulawa na ciki don reagents.

Tsarin Gwaji:

Da fatan za a karanta abin da aka saka kafin gwaji.

1. Ɗauki katin gwajin daga jakar takarda, sanya shi a kan teburin matakin kuma yi alama.

2.Cire hula daga tube samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 3 saukad (game da 100uL) babu kumfa diluted samfurin a tsaye da kuma sannu a hankali a cikin samfurin da kyau na katin tare da bayar dispette. Sannan fara mai ƙidayar lokaci.

3. Ya kamata a karanta sakamakon a cikin minti 10-15, kuma ba shi da inganci bayan minti 15.