Kit ɗin Gano don Progesterone (ƙimar fluorescence immunochromatographic)
Kit ɗin Diagnostic don Progesterone(Fluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.
AMFANI DA NUFIN
Kit ɗin Diagnostic don Progesterone (ƙaddamar da fluorescence immunochromatographic assay) shine gwajin immunochromatographic mai haske don gano ƙima na Progesterone (PROG) a cikin ƙwayar ɗan adam ko plasma, ana amfani dashi don ƙarin bincike na cututtukan cututtukan da ke da alaƙa da progesterone. . Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.
TAKAITACCEN
Progesterone wani muhimmin hormone ne wanda ke taka muhimmiyar rawa wajen daidaita yanayin haila kuma yana da mahimmanci wajen kiyaye ciki. Matsayin progesterone a cikin jini ya karu da sauri bayan ovulation. Alamar abin dogara ce ta yanayin kwai ko shigar da kwai.
KA'IDAR HANYA
An lulluɓe membrane na na'urar gwajin tare da haɗin BSA da PROG akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Alamar alamar an lullube ta da alamar fluorescence anti PROG antibody da zomo IgG a gaba. Lokacin gwada samfurin, PROG a cikin samfurin haɗe tare da alamar kyalli mai alamar antibody PROG, da samar da cakuda rigakafi. A karkashin aikin da immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun ya wuce gwajin yankin, The free kyalli alama za a hade tare da PROG a kan membrane.The maida hankali na PROG ne korau daidaituwa ga haske sigina, da kuma Ana iya gano maida hankali na PROG a cikin samfurin ta hanyar gwajin immunoassay fluorescence.
REAgents DA KAYAN DA AKA BAYAR
Abubuwan kunshin 25T:
Katin gwajin ɗai-ɗai wanda aka sanye da jakar 25T
Samfurin diluents 25T
Kunshin saka 1
KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci
MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabaru tattara samfurin. Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
3.All samfurin guje wa daskare-narke hawan keke.
HANYAR ASSAY
Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji.
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2Bude Portable Immune Analyzer(WIZ-A101), shigar da kalmar shiga ta asusun bisa ga tsarin aiki na kayan aiki, sa'annan ku shigar da wurin ganowa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6.Add 20μL serum ko plasma samfurin zuwa samfurin diluent, da kuma Mix da kyau ..
7.Add 80μL samfurin bayani don samfurin rijiyar katin.
8. Danna maɓallin "madaidaicin gwajin", bayan minti 10, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
9. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).
SAKAMAKON JARRABAWA DA FASSARA
Mataki | Rage (ng/ml) | |
Namiji | 0.1-0.9 | |
Mace | lokacin follicular / ovulatory period | 0.3-1.5 |
Lokaci na luteal | 5.2-18.5 | |
Menopause | 0.8 |
.Bayanan da ke sama shine sakamakon gwajin reagent na PROG, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa kewayon ƙimar gano PROG wanda ya dace da yawan jama'a a wannan yanki. Sakamakon da ke sama don tunani ne kawai.
.Sakamakon wannan hanyar yana aiki ne kawai ga jeri na tunani da aka kafa a cikin wannan hanyar, kuma babu kwatankwacin kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.
AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi. Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C. KAR KA DANKE. Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3Sample diluent ana amfani da shi nan da nan bayan an buɗe shi.
GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban No..
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.
LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin. Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA. Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, masu kula da marasa lafiya ya kamata a yi la'akari da su sosai tare da alamunta, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi ne kawai don gwajin jini da jini. Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.
HALAYEN YI
Linearity | 0.5ng/ml zuwa 50ng/ml | bambancin dangi: -15% zuwa +15%. |
Matsakaicin daidaitawar layi:(r)≥0.9900 | ||
Daidaito | Adadin dawowa zai kasance cikin 85% - 115%. | |
Maimaituwa | CV≤15% | |
Musamman(Babu ɗaya daga cikin abubuwan da aka gwada wanda aka gwada wanda ya tsoma baki a cikin binciken) | Tsangwama | Matsalolin tsaka-tsaki |
E2 | 500ng/ml | |
T | 500ng/ml | |
Kor | 500ng/ml | |
E3 | 100ng/ml | |
17 β-E2 | 100ng/ml |
REFERENCES
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.
Maɓalli ga alamomin da aka yi amfani da su:
In Vitro Diagnostic Medical Na'urar | |
Mai ƙira | |
Adana a 2-30 ℃ | |
Ranar Karewa | |
Kada a sake amfani | |
HANKALI | |
Tuntuɓi Umarnin Don Amfani |
Kudin hannun jari Xiamen Wiz Biotech Co.,Ltd
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