Kit ɗin bincike don Isoenzyme MB na Creatine Kinase(Fluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin Diagnostic don Isoenzyme MB na Creatine Kinase (ƙaddamarwar immunochromatographic assay) shine ƙirar immunochromatographic mai haske don gano ƙimar Isoenzyme MB na Creatine Kinase (CK-MB) a cikin ƙwayar ɗan adam ko plasma, ana amfani dashi don ƙarin bincike na AMI (Myocardial Myocardial). Ciwon ciki). Duk samfuran tabbatacce dole ne a tabbatar da su ta wasu hanyoyin. Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.

TAKAITACCEN
Matakan CK-MB sun karu sa'o'i 4-6 bayan ciwon zuciya na zuciya ya faru, ya kai kololuwar sa'o'i 18-24, kuma ya koma daidai kwanaki 2-3 daga baya. Sakin CK-MB zuwa wurare dabam dabam bayan haemoglobin.CK-MB shine alamar gargajiya ta myocardial infarction

KA'IDAR HANYA
An lulluɓe jikin na'urar gwajin da antibody CK-MB akan yankin gwajin da goat anti zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin anti CK-MB antibody da zomo IgG a gaba. Lokacin gwada ingantaccen samfurin, CK-MB antigen a cikin samfurin yana haɗuwa tare da walƙiya mai lakabin anti CK-MB antibody, kuma ya samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, shi hade da anti CK-MB shafi antibody, Forms sabon hadaddun. Matsayin CK-MB yana da alaƙa da alaƙa da siginar haske, kuma ana iya gano ƙaddamar da CK-MB a cikin samfurin ta hanyar gwajin immunoassay fluorescence.

REAgents DA KAYAN DA AKA BAYAR

Abubuwan kunshin 25T:
Katin gwajin ɗai-ɗai wanda aka sanye da jakar 25T
Samfurin diluents 25T
Kunshin saka 1

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabaru tattara samfurin. Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
3.All samfurin guje wa daskare-narke hawan keke.

HANYAR ASSAY
Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji.
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
5.Add 40μL serum ko plasma samfurin zuwa samfurin diluent, da kuma Mix da kyau ..
6.Add 80μL samfurin bayani don samfurin rijiyar katin.
7. Danna maɓallin "gwajin misali", bayan minti 15, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
8. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).

DABI'UN DA AKE SARANSU
CK-MB <5ng/ml
Ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa nasa kewayon al'ada wanda ke wakiltar yawan majinyacinsa.

SAKAMAKON JARRABAWA DA FASSARA
.Bayanan da ke sama sakamakon CK-MB reagent gwajin ne, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa kewayon ƙimar gano CK-MB wanda ya dace da al'ummar wannan yanki. Sakamakon da ke sama don tunani ne kawai.
.Sakamakon wannan hanyar yana aiki ne kawai ga jeri na tunani da aka kafa a cikin wannan hanyar, kuma babu kwatankwacin kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.

AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi. Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C. KAR KA DANKE. Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.

GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban No..
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.

LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin. Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA. Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, masu kula da marasa lafiya ya kamata a yi la'akari da su sosai tare da alamunta, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi ne kawai don gwajin jini da jini. Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.

HALAYEN YI

REFERENCES
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.

Maɓalli ga alamomin da aka yi amfani da su:

	In Vitro Diagnostic Medical Na'urar
	Mai ƙira
	Adana a 2-30 ℃
	Ranar Karewa
	Kada a sake amfani
	HANKALI
	Tuntuɓi Umarnin Don Amfani

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