Don a cikin amfani da bincike na vitro kawai

Da fatan za a karanta wannan kunshin a hankali kafin amfani da kuma bin umarnin sosai. Amincewa da sakamakon assay ba zai yiwu ba idan akwai wasu karkacewa daga umarnin a cikin wannan kunshin sakawa.

Amfani da aka yi niyya

Kit ɗin bincike don hepatitis c kwayar cuta ta cheustomody (fitsari imminakalcic assay) ƙimar bincike na yau da kullun don kamuwa da cutar hepatitis C.LL ne hanyoyin. Wannan gwajin ya yi niyyar amfani da shi ne kawai

1.Ya ba duk reagents da samfurori zuwa zazzabi ba.
2. Ween da aka ɗaukuwa mai ɗaukar rigakafi na rigakafi (Wiz-A101), shigar da kalmar wucewa ta asusun shiga gwargwadon hanyar aikin na kayan aiki, kuma shigar da ganowa.
3.Can lambar hakori don tabbatar da abun gwajin.
4.Ka fitar da katin gwajin daga jakar tsare.
5. Areasarin katin gwajin a cikin slot, bincika lambar QR, kuma ƙayyade abu na gwajin.
6.dd 20μl serum ko plasma samfurin zuwa samfurin mai dorulewa, kuma Mix da kyau ..
7.Add samfurin 80μl samfurin don samfurin da kyau na katin.
8.Click da "Standard Gwaji", bayan mintina 15, kayan aikin zai iya gano katin gwajin ta atomatik, da rikodin / buga sakamakon gwajin.
9.ReFer zuwa ga umarnin mai amfani na rigakafi (WIZ-A101).

Taƙaitawa

Cutar kwayar cuta (HCV) ambulaf ne, aure da aka daidaita da hankali RNA (9.5 KB) Virus na dangin Flaviviriidae. An gano manyan manyan halittar dabbobi shida da jerin magudi na HCV. An ware a 1989, yanzu an amince da HCV a matsayin babban dalilin shigowa da ke da alaƙa da, wanda ba a B hepatitis ba. Cutar tana da halin m da na kullum tsari. Fiye da kashi 50% na mutane masu kamuwa da cutar suna haɓaka mai tsanani, barazanar rayuwa na fuskantar matsalar hepatitis tare da hanta cirrhososomas. Tun bayan gabatarwar a shekarar 1990 na Anti-HCV na gudummawar jini, abin da ya faru da wannan kamuwa da cuta a cikin masu karɓa masu karɓa an rage su sosai. Nazarin Clinical ya nuna cewa gagarumar adadin mutane na cutar HCV suna haɓaka abubuwan rigakafi zuwa NS5 wanda ba tsarin ƙwayar cuta ba. A saboda wannan, gwaje-gwajen sun hada da Antigens daga yankin NS5 na naúrar ta hannu ta hanyar NS3 (C200), NS4 (C200) da Core (C22).

Ka'idar aikin

An yi amfani da membrane na na'urar gwajin tare da HCV Antigen a kan yankin gwaji da akuya ta hana zomo Igg a kan ikon sarrafawa. An rufe allon lloresceny mai haske ta hanyar kyalli mai kyau da zomo antigg a gaba. A lokacin da gwada ingantaccen samfurin, da HCV Daliban a cikin samfurin haɗuwa tare da mai kyalli mai haske, kuma samar da cakuda na rigakafi. A ƙarƙashin aikin immunochromatographymraphy, da hadaddun kwarara ne a cikin shugabanci na neman antigen, ya hada da sabon hadadden na HCV da aka haɗa da siginar gwaji, kuma taro na Ana iya gano HCV a cikin samfurin ta hanyar kyalli Imunoassay Assayi

Reagents da kayan da aka kawo

25t kunshin kunshin:
.Test katin daban-daban tsare a cikin desiccant
.Sample dilun
. Saka

Abubuwan da ake buƙata amma ba a bayar ba
Sample tarin, lokaci
Sample tattara da adanawa
1.Wane samfuran gwajin zai iya zama magani, Heparin anticiagulant plasma ko edta edicoulant plasma.
2.Akboring zuwa daidaitattun fasahohin tattara samfuri. Serum ko samfurin plasma za a iya kiyaye firiji a 2-8 ℃ don 7days da cryopreoss ƙasa -15 ° C na watanni 6
3.all samfurin Guji gujewa 'narke-thaw hycles.

Tsarin Assay
Da fatan za a karanta aikin kayan aiki da aka shigar da kunshin kaya kafin gwaji.
.This sakamakon gwajin asibiti ne kawai don tunani ne kawai, bai kamata ya zama tushen kawai ga asalin cutar ta asibiti ba, amsar marasa bincike, amsawar marasa lafiya, amsar marasa lafiya da sauran bayanan .
.This sake amfani da shi ne kawai don magani da gwajin plasma. Yana iya samun cikakken sakamako lokacin amfani da wasu samfurori kamar siliki da fitsari da sauransu.

Halaye na aiki

Nassoshi
1. A: Moore SB, Ed. Transfuson-da aka watsa da cututtukan hoto ko bidiyo mai zagaya yanar gizo. Alington, va. Ni. M. Bankunan jini, PP. 53-38.
2.Hansen Jh, et Al.ham Comments tare da Murine Monogoasals antawun immunoassays [j] .j immanmoasy
3.Llevinson ss.The yanayin halittu na heterophilic da rawar da ke cikin incunoassay [J] .j imminoasay [1992,14: 108-114.
4.Alter HJ., Sufell RH, Holland Pv, et al. (1978) wakili mai ba da izini a cikin ba a, ba-b hepatitis. Lancet i: 459-463.
5.Choo QL, Weiner Aj, weiner lr lr lr lr lr, kuo G, Hepatitis C kwayar cuta mara kyau: Babban wakili na mara-sauri. Br m m m m mura 46: 423-441.
6.ENGVALL E, Perlmann P. (1971) enzyme da ke da alaƙa da rigakafin amintaccen (Elisa): Assadative Assayi na Igg. Immunochemarwa 8: 871-874.

Abubuwan da ake tsammani

Hcv-ab <0.02

An ba da shawarar cewa kowane dakin gwaje-gwaje yana kafa kewayon na al'ada mai wakiltar yawan masu haƙuri.

Sakamakon gwajin da fassara

• Bayanin da ke sama shine sakamakon gwajin HCV-HB, kuma ya ba da shawarar kowane dakin gwaje-gwaje ya dace da yawan dabi'un gano HCV-Abs dace da yawan halaye a wannan yankin. Sakamakon da ke sama shine kawai don tunani kawai.
• Sakamakon wannan hanyar ana zartar da kawai ga jerin abubuwan nuni da aka kafa ta wannan hanyar, kuma babu wani hatsari tare da sauran hanyoyin.
• Sauran dalilai na iya haifar da kurakurai a sakamakon ganowa, gami da dalilai na fasaha, kurakurai aiki da sauran samfurin samfurin.

Adana da kwanciyar hankali

1. Kit ɗin shine watanni 18-rayuwa daga ranar samarwa. Adana kayan da ba a amfani da su a 2-30 ° C. Kada ku daskare. Kar a yi amfani da ranar karewa.
2. Kada ku buɗe jikkunan da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar gwajin amfani a ƙarƙashin yanayin da ake buƙata (zafi 40-90%) a cikin mintuna 60 da sauri .
3. Ana amfani da samfurin da aka yi amfani da shi nan da nan bayan an buɗe.

Gargadi da Gargadi
Ya kamata a rufe kit ɗin kuma a kiyaye shi da danshi.
.Layan samfurori zai iya amfani da wasu hanyoyin.
.Za a kula da dukkan samfuri a matsayin yiwuwar ƙazanta.
.Kada ku yi amfani da mai dainiyar da aka ƙare.
.Kar a canza reagents tsakanin kits tare da da yawa ba ..
.Kada kuyi amfani da katunan gwaji da kowane kayan haɗi masu zazzagewa.
.Rashin daidaituwa, wuce gona da iri ko kadan samfurin zai haifar da rikicewar sakamako.

LKwaikwayo
.Kamar yadda yake tare da kowane irin aiki na yin amfani da kayan linzamin kwamfuta, yiwuwar kasancewa mai tsoma baki ta maganin hana haifuwa (Hama) a cikin samfuran. Mane samfurori daga marasa lafiya waɗanda suka sami shirye-shirye na abubuwan rigakafi na moniclonal don ganewar asali ko farjin na iya ƙunsar Hama. Irin waɗannan samfurori na iya haifar da ingantaccen sakamako na ƙarya.
Mabuɗin kan alamomin da aka yi amfani da su:

	A cikin na'urar kiwon lafiya na Vitro
	Mai masana'anta
	Store a 2-30 ℃
	Ranar karewa
	Kada ku sake amfani
	Hankali
	Taimaka wa umarnin don amfani