Kit ɗin bincike don Troponin na Cardiac I (ƙimar immunochromatographic fluorescence)
Kit ɗin bincike don cardiac Troponin I(Fluorescence immunochromatographic assay)
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.
AMFANI DA NUFIN
Kit ɗin bincike don Troponin na Cardiac Troponin I (ƙimar fluorescence immunochromatographic assay) shine gwajin immunochromatographic fluorescence don ƙididdigar ƙididdigewa na Cardiac Troponin I (cTnI) a cikin ƙwayar ɗan adam ko plasma, ana amfani dashi don ƙarin bincike na AMI (Myocardial Infarction). Duk samfuran tabbatacce dole ne a tabbatar da su ta wasu hanyoyin. Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai.
TAKAITACCEN
Matakan cTnI sun karu sa'o'i da yawa bayan ciwon zuciya na zuciya ya faru, ya kai kololuwa a sa'o'i 12-16, kuma ya kasance daga sama kwanaki 4-9 bayan ciwon bugun zuciya ya faru. Ma'anar duniya game da ciwon zuciya na uku a cikin 2012: Mafi kyawun biomarker-cTn (I ko T), yana da ƙayyadaddun nama na tsokar zuciya da haɓakar asibiti. Canje-canje a cikin maida hankali na cTn suna da mahimmanci ga ganewar asali na AMI
KA'IDAR HANYA
An lulluɓe jikin na'urar gwajin da anti-cTnI antibody akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar fluorescence mai lakabin anticTnI antibody da zomo IgG a gaba. Lokacin gwada ingantaccen samfurin, cTnI antigen a cikin samfurin yana haɗuwa tare da mai walƙiya mai lakabin anticTnI antibody, kuma ya samar da cakuda rigakafi. A karkashin aikin na immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, shi a hade tare da anti-cTnI shafi antibody, Forms sabon complex.cTnI matakin da gaskiya da dangantaka da haske sigina, da kuma maida hankali na cTnI. a cikin samfurin za a iya gano ta hanyar fluorescence immunoassay assay.
REAgents DA KAYAN DA AKA BAYAR
Abubuwan kunshin 25T:
Katin gwajin ɗai-ɗai wanda aka sanye da jakar 25T
Samfurin diluents 25T
Kunshin saka 1
KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
Samfurin tarin akwati, mai ƙidayar lokaci
MISALI TATTAUNAWA DA AJIYA
1. Samfuran da aka gwada na iya zama jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2.According ga daidaitattun dabaru tattara samfurin. Za'a iya adana ruwan magani ko samfurin plasma a cikin firiji a 2-8 ℃ na tsawon kwanaki 7 kuma ana kiyaye cryopreservation ƙasa -15 ° C na watanni 6.
3.All samfurin guje wa daskare-narke hawan keke.
HANYAR ASSAY
Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji.
1.Lay a gefe duk reagents da samfurori zuwa dakin zafin jiki.
2.Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar sirri shiga asusu bisa ga tsarin aiki na kayan aiki, da kuma shigar da ganowa dubawa.
3.Scan da lambar haƙori don tabbatar da gwajin abu.
4.Dauki katin gwaji daga jakar foil.
5.Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6.Add 40μL serum ko plasma samfurin zuwa samfurin diluent, da kuma Mix da kyau ..
7.Add 80μL samfurin bayani don samfurin rijiyar katin.
8. Danna maɓallin "gwajin misali", bayan minti 15, kayan aiki za su gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma rikodin / buga sakamakon gwajin.
9. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).
DABI'UN DA AKE SARANSU
cTnI <0.3ng/ml
Ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa nasa kewayon al'ada wanda ke wakiltar yawan majinyacinsa.
SAKAMAKON JARRABAWA DA FASSARA
.Bayanan da ke sama sakamakon gwajin reagent na cTnI ne, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kafa kewayon ƙimar gano cTnI wanda ya dace da yawan jama'a a wannan yanki. Sakamakon da ke sama don tunani ne kawai.
.Sakamakon wannan hanyar yana aiki ne kawai ga jeri na tunani da aka kafa a cikin wannan hanyar, kuma babu kwatankwacin kai tsaye da sauran hanyoyin.
.Wasu dalilai kuma na iya haifar da kurakurai a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakuran aiki da sauran abubuwan samfuri.
AJIYA DA KWANTA
1.The kit ne 18 watanni shiryayye-rai daga ranar da aka yi. Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C. KAR KA DANKE. Kar a yi amfani da bayan ranar karewa.
2.Kada ku buɗe jakar da aka rufe har sai kun shirya don yin gwaji, kuma ana ba da shawarar yin amfani da gwajin amfani ɗaya a ƙarƙashin yanayin da ake buƙata (zazzabi 2-35 ℃, zafi 40-90%) a cikin 60 mins da sauri. kamar yadda zai yiwu.
3.Sample diluent ana amfani da shi nan da nan bayan an bude shi.
GARGADI DA TSIRA
.Kit ɗin yakamata a rufe shi kuma a kiyaye shi daga danshi.
.Duk samfurori masu inganci za a inganta su ta wasu hanyoyin.
.Duk samfuran za a kula da su azaman masu gurɓata yanayi.
.KADA KA yi amfani da reagent da ya ƙare.
.KADA KA musanya reagents tsakanin kits tare da kuri'a daban-daban No..
.KADA KA sake amfani da katunan gwaji da duk wani kayan haɗi da za'a iya zubarwa.
.Misoperation, wuce kima ko kadan samfurin iya haifar da sabawa sakamakon.
LIMITATION
Kamar yadda yake tare da kowane gwajin amfani da ƙwayoyin rigakafi na linzamin kwamfuta, akwai yuwuwar kutsawa daga ƙwayoyin rigakafin linzamin kwamfuta na ɗan adam (HAMA) a cikin samfurin. Samfura daga marasa lafiya waɗanda suka karɓi shirye-shiryen rigakafin ƙwayoyin cuta na monoclonal don ganewar asali ko jiyya na iya ƙunsar HAMA. Irin waɗannan samfurori na iya haifar da sakamako mara kyau na ƙarya ko ƙarya.
.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, masu kula da marasa lafiya ya kamata a yi la'akari da su sosai tare da alamunta, tarihin likita, sauran gwaje-gwaje na dakin gwaje-gwaje, amsawar magani, cututtukan cututtuka da sauran bayanai. .
.Wannan reagent ana amfani dashi ne kawai don gwajin jini da jini. Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.
HALAYEN YI
| Linearity | 0.1ng/ml zuwa 40ng/ml | bambancin dangi: -15% zuwa +15%. |
| Matsakaicin daidaitawar layi:(r)≥0.9900 | ||
| Daidaito | Adadin dawowa zai kasance cikin 85% - 115%. | |
| Maimaituwa | CV≤15% | |
| Musamman(Babu ɗaya daga cikin abubuwan da aka gwada wanda aka gwada wanda ya tsoma baki a cikin binciken) | Tsangwama | Matsalolin tsaka-tsaki |
| sTnI | 1000μg/L | |
| cTnT | 1000μg/L | |
| ABP | 1000μg/L | |
| CK-MB | 1000μg/L | |
| cTnC | 1000μg/L | |
| sTnT | 1000μg/L | |
| MYO | 1000μg/L | |
REFERENCES
1.Hansen JH, et al.HAMA Tsangwama tare da Murine Monoclonal Antibody-Based Immunoassays[J].J na Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Yanayin Heterophilic Antibodies da Matsayin Tsangwama na Immunoassay[J].J na Clin Immunoassay,1992,15:108-114.
Maɓalli ga alamomin da aka yi amfani da su:
 | In Vitro Diagnostic Medical Na'urar |
 | Mai ƙira |
 | Adana a 2-30 ℃ |
 | Ranar Karewa |
 | Kada a sake amfani |
 | HANKALI |
 | Tuntuɓi Umarnin Don Amfani |
Kudin hannun jari Xiamen Wiz Biotech Co.,Ltd
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