Ka'idodin bincike na bitamin 25-hydroxy bitamin d 25(impescence immunochromogographic ass)
Don a cikin amfani da bincike na vitro kawai
Da fatan za a karanta wannan kunshin a hankali kafin amfani da kuma bin umarnin sosai. Amincewa da sakamakon assay ba zai yiwu ba idan akwai wasu karkacewa daga umarnin a cikin wannan kunshin sakawa.

Amfani da aka yi niyya
Ka'idodin bincike na bitamin 25-hydroxy na bitamin 25 (mai haske mai haske immunogographic assay) a cikin maganin gargajiya ko plasma, wanda aka yi amfani da shi don kimanta matakan na bitamin D.it wani abu ne mai ganowa na taimako na taimako. Shin ingantaccen samfurin dole ne a tabbatar da wasu hanyoyin. Wannan gwajin ya yi niyyar amfani da shi ne kawai.

Taƙaitawa
Vitamin D shine bitamin kuma shi ma steroid mai steroid ne, mafi yawa ciki har da VD3 da VD3, wanda ɓarna yake kama da haka. Vitamin D3 da D2 suna canzawa zuwa Vitamin 25 Hydroxyl D (ciki har da 25-D2 D2 D2). 25- (oh) vd a jikin mutum, fitina mai rauni, babban taro. 25- (oh) vd yana nuna jimlar adadin bitamin D, da kuma iyawar bitamin D, don haka 25- (oh) VD ana ɗauka shine mafi kyawun misalin bitamin D.The maganin bincike ya dogara da shi immunochromatic kuma zai iya bada sakamako a cikin mintina 15.

Ka'idar aikin
A membrane na na'urar gwajin yana da alaƙa da conjugate na BSA da 25- (oh) VD akan Yankin Gwada da Goat Antibden engg a kan ikon sarrafawa. Ana mai da alamar songer ta hanyar kyalli Mark Anti 25- (oh) vd antibody da zomo Igg a gaba. A lokacin da aka gwada samfurin, 25- (oh) vd a cikin samfurin haɗuwa tare da alamar kyalli mai launin anti 25- (oh) vd antiby, kuma samar da cakuda na rigakafi. A ƙarƙashin aikin immunochromatographyoch, hadadden kwarara a cikin shugabanci na gwiwowi, (oh) vd a kan membrane. (oh) vd a kan membrane. (oh) VD mummunan abu ne mara kyau ga siginar kyalli, da kuma taro na 25- (oh) vd a cikin samfurin za'a iya gano su ta hanyar kyamarwar imminoassay Assoresceny.

Reagents da kayan da aka kawo

25t kunshin kunshin:
.Test katin daban-daban tsare ya pouched da desiccant 25t
.A bayani na 25T
.B bayani 1
.Pawing saka 1

Abubuwan da ake buƙata amma ba a bayar ba
Sample tarin, lokaci

Sample tattara da adanawa
1.Wane samfuran gwajin zai iya zama magani, Heparin anticiagulant plasma ko edta edicoulant plasma.
2.Akboring zuwa daidaitattun fasahohin tattara samfuri. Serum ko samfurin plasma za a iya kiyaye sanyaya a 2-8 ℃ don 7days da cryopreoss ƙasa -15 ° C na watanni 6.
3.all samfurin Guji gujewa 'narke-thaw hycles.

Tsarin Assay
Hanyar gwajin na kayan aiki suna ganin manjojin rigakafi. Tsarin sake gwadawa shine kamar haka
1.Ya ba duk reagents da samfurori zuwa zazzabi ba.
2. Ween da aka ɗaukuwa mai ɗaukar rigakafi na rigakafi (Wiz-A101), shigar da kalmar wucewa ta asusun shiga gwargwadon hanyar aikin na kayan aiki, kuma shigar da ganowa.
3.Can lambar hakori don tabbatar da abun gwajin.
4.Ka fitar da katin gwajin daga jakar tsare.
5. Areasarin katin gwajin a cikin slot, bincika lambar QR, kuma ƙayyade abu na gwajin.
6.Dd 30μl Serum ko plasma samfurin zuwa mafita, kuma Mix da kyau.
7.Ad 50μl B bayani zuwa cakuda da ke sama, kuma Mix da kyau.
8.Bar cakuda na mintina 15.
9.Add cakuda zuwa samfurin kyau na katin.
10.Click da "Standard Gwajin Gwaji", bayan mintuna 10, kayan aikin zai iya gano katin gwajin ta atomatik, da rikodin / buga sakamakon gwajin.
11.Refer zuwa ga umarnin nazarin rigakafi na irly (WIZ-A101).

Abubuwan da ake tsammani
25- (oh) vd al'ada ta al'ada: 30-100ng / ml
An ba da shawarar cewa kowane dakin gwaje-gwaje yana kafa kewayon na al'ada mai wakiltar yawan masu haƙuri.

Sakamakon gwajin da fassara
.The sama bayanai shine tazara tazara ta hanyar gano bayanan wannan katun, kuma ya kamata kowane dakin gwaje-gwaje mahimmancin m mahimmancin wannan yankin.
.Ka dawowar 25- (Oh) VD ya fi kewayon zance da kewayon magana, kuma ya kamata a cire canje-canje na jiki ko kuma amsar damuwa.
.The sakamakon wannan hanyar ana zartar da kawai ga kewayon zango ta hanyar wannan hanyar, kuma sakamakon ba tare da sauran hanyoyin ba.
Hakanan na iya haifar da kuskure a cikin sakamakon ganowa, gami da dalilai na fasaha, kurakurai aiki da sauran samfuran samfurin.

Adana da kwanciyar hankali
.The Kit shine watanni 18-rayuwarsa daga ranar samarwa. Adana kayan da ba a amfani da su a 2-30 ° C. Kada ku daskare. Kar a yi amfani da ranar karewa.
.Ka buɗe aljihunan da aka rufe har sai ka shirya don yin gwaji, kuma ana ba da shawarar gwajin guda ɗaya a ƙarƙashin mintuna 6-90%) cikin sauri mai yiwuwa.
.Amma ana amfani da dorriple nan da nan bayan an buɗe.

Gargadi da Gargadi
Ya kamata a rufe kit ɗin kuma a kiyaye shi da danshi.
.Layan samfurori zai iya amfani da wasu hanyoyin.
.NA Za a kula da samfuran samfurori azaman yuwuwar ƙazanta.
.Da ba amfani da mai ƙarewa ba.
.Do ba musayar reagals tsakanin kits tare da daban-daban ba ..
.Ka sake yin amfani da katunan gwaji da kowane kayan haɗi masu zazzagewa.
.Muiseoperation, wuce gona da iri ko kadan samfurin zai haifar da jujjuyawar sakamako.

LKwaikwayo
.As tare da kowane irin aiki suna amfani da kayan aikin linzamin kwamfuta, yiwuwar rayuwa don tsoma baki (Hama) a cikin samfuran. Mane samfurori daga marasa lafiya waɗanda suka sami shirye-shirye na abubuwan rigakafi na moniclonal don ganewar asali ko farjin na iya ƙunsar Hama. Irin waɗannan samfurori na iya haifar da ingantaccen sakamako na ƙarya.
.This sakamakon gwajin asibiti ne kawai don tunani ne kawai, bai kamata ya zama tushen kawai ga asalin cutar ta asibiti ba, amsar marasa bincike, amsawar marasa lafiya, amsar marasa lafiya da sauran bayanan .
.This sake amfani da shi ne kawai don magani da gwajin plasma. Yana iya samun cikakken sakamako lokacin amfani da wasu samfurori kamar siliki da fitsari da sauransu.

Halaye na aiki

RDaure

1.Hansen Jh, et Al.ham Comments tare da Murine Monogoassays [J]
2.levinson ss.The yanayin halittu na heterophilic da kuma rawar da ke cikin imnunoassay [J] .j imminoasay [1992,14: 108-114.

Mabuɗin kan alamomin da aka yi amfani da su:

	A cikin na'urar kiwon lafiya na Vitro
	Mai masana'anta
	Store a 2-30 ℃
	Ranar karewa
	Kada ku sake amfani
	Hankali
	Taimaka wa umarnin don amfani

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