KA'IDA

Ana rufe membrane na na'urar gwajin tare da haɗin BSA da 25- (OH) VD akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Alamar alamar ana lullube ta da alamar fluorescence anti 25-(OH) VD antibody da zomo IgG a gaba. Lokacin gwajin samfurin, 25- (OH) VD a cikin samfurin haɗa tare da mai walƙiya alama anti 25- (OH) VD antibody, da kuma samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda, a lokacin da hadaddun wuce gwajin yankin, The free kyalli alama za a hade tare da 25- (OH) VD a kan membrane.The taro na 25- (OH) VD ba daidai ba ne don siginar haske, kuma ƙaddamar da 25- (OH) VD a cikin samfurin za a iya gano shi ta hanyar gwajin immunoassay fluorescence.

Tsarin Gwaji:

Da fatan za a karanta jagorar aiki na kayan aiki da saka fakiti kafin gwaji.

1. Ajiye duk reagents da samfurori zuwa zafin jiki.
2. Bude Portable Immune Analyzer (WIZ-A101), shigar da kalmar wucewa ta asusun bisa ga tsarin aiki na kayan aiki, kuma shigar da hanyar ganowa.
3. Duba lambar haƙori don tabbatar da abin gwajin.
4. Fitar da katin gwajin daga jakar jakar.
5. Saka katin gwaji a cikin ramin katin, duba lambar QR, kuma ƙayyade abin gwajin.
6. Ƙara 15μL ruwan magani ko samfurin plasma a cikin bayani, kuma haɗuwa da kyau
7. Ƙara cakuda 80μL zuwa samfurin rijiyar katin.
8. Danna maballin "standard test", bayan minti 15, na'urar za ta gano katin gwajin ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aiki, kuma ya rubuta / buga sakamakon gwajin.
9. Koma zuwa ga umarnin Portable Immune Analyzer (WIZ-A101).