Kit ɗin bincike(Colloidal Gold)don Calprotectin
Don bincikar in vitro amfani kawai
Da fatan za a karanta wannan fakitin a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da amincin sakamakon kima ba idan akwai wasu sabani daga umarnin a cikin wannan fakitin.

AMFANI DA NUFIN
Kit ɗin bincike don Calprotectin (cal) shine gwajin immunochromatographic na zinari don ƙididdige ƙididdiga na cal daga najasar ɗan adam, wanda ke da mahimmancin ƙima na kayan bincike don cututtukan hanji mai kumburi. Wannan gwajin reagent ne na dubawa. Duk samfuran tabbatacce dole ne a tabbatar da su ta wasu hanyoyin. Anyi nufin wannan gwajin don amfanin ƙwararrun kiwon lafiya kawai. A halin yanzu, ana amfani da wannan gwajin don IVD, ƙarin kayan aikin ba a buƙata.

TAKAITACCEN
Cal shine heterodimer, wanda ya ƙunshi MRP 8 da MRP 14. Yana wanzu a cikin cytoplasm neutrophils kuma an bayyana akan membranes cell mononuclear. Cal sunadaran sunadaran lokaci ne, yana da kwanciyar hankali kamar mako guda a cikin najasar ɗan adam, an ƙaddara ya zama alamar cutar hanji mai kumburi. Kit ɗin gwaji ne mai sauƙi, na gani na zahiri wanda ke gano cal a cikin najasar ɗan adam, yana da haɓakar ganowa da ƙaƙƙarfan ƙayyadaddun bayanai. Gwajin da aka danganta da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin rigakafin ƙwayoyin cuta guda biyu na sanwichi amsawa da fasahar bincike na gwajin immunochromatographic na gwal, yana iya ba da sakamako cikin mintuna 15.

KA'IDAR HANYA
Tushen yana da maganin rigakafin ƙwayar cuta McAb akan yankin gwaji da goat anti-zomo IgG antibody akan yankin sarrafawa, wanda aka liƙa zuwa membrane chromatography a gaba. Label pad an lullube shi da zinari mai launi mai lakabin anti cal McAb da kuma zinare mai alamar zomo IgG antibody a gaba. Lokacin da aka gwada tabbataccen samfurin, cal a cikin samfurin ya zo tare da colloidal zinariya mai lakabin anti cal McAb, kuma ya zama hadaddun rigakafi, yayin da aka ba shi izinin yin ƙaura tare da tsiri na gwaji, hadaddun cal conjugate an kama shi da maganin antical McAb akan membrane da sifa. "Anti cal coating McAb-cal-colloidal zinariya mai lakabin anti cal McAb" hadaddun, wani launi na gwaji ya bayyana a yankin gwaji. Ƙarfin launi yana da alaƙa daidai da abun ciki na cal. Samfura mara kyau baya samar da band ɗin gwaji saboda rashin haɗin gwal na haɗin gwal na colloidal. Komai cal ya kasance a cikin samfur ko a'a, akwai jajayen ratsin da ke bayyana akan yankin tunani da yankin sarrafa inganci, wanda ake ɗaukarsa azaman ingantattun ma'auni na kasuwanci na ciki.

REAgents DA KAYAN DA AKA BAYAR
Abubuwan kunshin 25T:
.Katin gwajin ɗaiɗaiku foil ɗin da aka shayar da shi tare da na'urar bushewa
.Sample diluents: sinadaran ne 20mM pH7.4PBS
.Rarraba
. Kunshin saka

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA

Samfurin tarin akwati, mai ƙidayar lokaci

MISALI TATTAUNAWA DA AJIYA
Yi amfani da akwati mai tsaftar da za a iya zubarwa don tattara samfurin najasa sabo, kuma a gwada nan da nan. Idan ba za a iya gwadawa nan da nan ba, da fatan za a adana a 2-8 ° C na awanni 12 ko ƙasa -15 ° C na tsawon watanni 4.

HANYAR ASSAY
1.Za a fitar da sandar samfurin, a saka a cikin samfurin najasa, sannan a mayar da sandar samfurin baya, a dunƙule sosai kuma a girgiza sosai, maimaita aikin sau 3. Ko kuma amfani da samfurin manne kamar 50mg na najasar samfurin, sannan a saka a cikin bututun samfurin najasa mai ɗauke da samfurin dilution, sannan a dunƙule tam.
2.Yi amfani da samfurin pipette da za a iya zubar da shi, ɗauki samfurin najasar ƙanƙara daga mai ciwon zawo, sannan a ƙara digo 3 (kimanin 100uL) a cikin bututun samfur ɗin fecal sannan a girgiza sosai, a ajiye.
3. Ɗauki katin gwajin daga jakar takarda, sanya shi a kan teburin matakin kuma yi alama.
4.Cire hula daga tube samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 3 saukad da (game da 100uL) babu kumfa diluted samfurin a tsaye da kuma sannu a hankali a cikin samfurin da kyau na katin tare da bayar dispette, fara lokaci.
5.Ya kamata a karanta sakamakon a cikin minti 10-15, kuma ba shi da inganci bayan minti 15.

SAKAMAKON JARRABAWA DA FASSARA

AJIYA DA KWANTA
Kit ɗin shine tsawon rayuwar watanni 24 daga ranar da aka yi. Ajiye kayan da ba a yi amfani da su ba a 2-30 ° C. Kar a bude jakar da aka hatimi har sai kun shirya yin gwaji.
GARGADI DA TSIRA
1.Kit ɗin ya kamata a rufe shi kuma a kare shi daga danshi¹.
2.Kada ku yi amfani da samfurin da aka sanya dogon lokaci ko maimaita daskarewa da narke don gwadawa
3.Fecal samfurori ya wuce kima ko kauri na iya yin samfurin da aka lalatar da katin gwaji mara kyau, don Allah centrifuge samfurin diluted kuma ɗauki supernatant don gwaji.
4.Misoperation, wuce kima ko ƙananan samfurin zai iya haifar da rarrabuwa sakamakon.

IYAKA
1.Wannan sakamakon gwajin shine kawai don tunani na asibiti, bai kamata ya zama tushen kawai don ganewar asibiti da magani ba, yakamata a yi la'akari da kulawar marasa lafiya tare da alamunta, tarihin likita, sauran binciken dakin gwaje-gwaje, amsawar jiyya, cututtukan cututtuka da sauran su. bayani².
2.Wannan reagent ana amfani dashi kawai don gwaje-gwaje na fecal. Maiyuwa baya samun ingantaccen sakamako idan aka yi amfani da shi don wasu samfura kamar yau da fitsari da sauransu.