KA'IDA

An lulluɓe membrane na na'urar gwajin da Tf antibody akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin anti Tf antibody da zomo IgG a gaba. Lokacin gwada ingantaccen samfuri, Tf antigen a cikin samfurin yana haɗuwa tare da walƙiya mai lakabin anti Tf antibody, kuma ya samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda. Lokacin da hadaddun ya wuce yankin gwajin, haɗe shi da anti-Tf shafi antibody, ya haifar da sabon hadaddun.

Idan ba shi da kyau, samfurin ba ya ƙunshi transferrin ko abun ciki yana da ƙananan, don haka ba za a iya kafa hadaddun rigakafi ba. Ba za a sami jan layi a wurin ganowa ba (T). Ko da ko Tf antibody ya wanzu a cikin samfurin ko a'a, ragowar colloidal zinare mai lakabin linzamin kwamfuta anti-human IgM monoclonal antibody da goat anti-mouse IgG antibody mai rufi a cikin layin kula da inganci (C). Sa'an nan agglutinates suna haɓaka launi a cikin yankin kula da inganci, kuma layin ja zai bayyana a cikin (C). Layin ja shine ma'auni yana bayyana a cikin yankin kula da inganci (C) don yin hukunci ko akwai isassun samfurori kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani dashi azaman ma'aunin kulawa na ciki don reagents.

Tsarin Gwaji

Da fatan za a karanta abin da aka saka kafin gwaji.

1. Ɗauki katin gwajin daga jakar takarda, sanya shi a kan teburin matakin kuma yi alama.

2.Cire hula daga tube samfurin kuma jefar da na farko biyu saukad da diluted samfurin, ƙara 3 saukad (game da 100uL) babu kumfa diluted samfurin a tsaye da kuma sannu a hankali a cikin samfurin da kyau na katin tare da bayar dispette. Sannan fara mai ƙidayar lokaci.