KA'IDA

Ana lulluɓe da membrane na na'urar gwajin tare da antigen Rotavirus Group A akan yankin gwajin da goat anti-zomo IgG antibody akan yankin sarrafawa. Label pad an lullube shi ta hanyar walƙiya mai lakabin anti Rotavirus Group A da zomo IgG a gaba. Lokacin gwada ingantaccen samfurin, RV a cikin samfurin yana haɗuwa tare da walƙiya mai lakabin anti Rotavirus Group A, kuma ya samar da cakuda rigakafi. A karkashin aikin immunochromatography, da hadaddun ya kwarara a cikin shugabanci na absorbent takarda. Lokacin da hadaddun ya wuce yankin gwajin, haɗe shi da anti-Rotavirus Group A antibody, yana samar da sabon hadaddun. Idan ba shi da kyau, babu antigen Rotavirus Group A a cikin samfurin, ta yadda ba za a iya samar da hadaddun garkuwar jiki ba, ba za a sami jan layi a wurin ganowa ba (T). Ko da ko rukuni na A rotavirus yana cikin samfurin, linzamin kwamfuta mai lakabin latex IgG an yi masa chromatographed zuwa yankin kula da inganci (C) kuma an kama shi ta anti-mouse IgG antibody. Jajayen layi zai bayyana a yankin kula da inganci (C). Layin ja shine ma'auni yana bayyana a cikin yankin kula da inganci (C) don yin hukunci ko akwai isassun samfurori kuma ko tsarin chromatography na al'ada ne. Hakanan ana amfani dashi azaman ma'aunin kulawa na ciki don reagents.

Tsarin Gwaji:

1.Ya kamata a tattara marasa lafiya na alamomi. A cewar rahotanni, matsakaicin fitar da rotavirus a cikin najasar marasa lafiya tare da gastroenteritis yana faruwa kwanaki 3-5 bayan fara cutar da kwanaki 3-13 bayan fara bayyanar cututtuka. Idan an tattara samfurin tsawon lokaci bayan zawo, adadin antigens bazai isa ya haifar da sakamako mai kyau ba.

2.Ya kamata a tattara samfurori a cikin tsabta, bushe, ruwa mai tsabta wanda ba ya ƙunshi kayan wankewa da masu kiyayewa.

3.Don marasa lafiya marasa lafiya, samfuran najasar da aka tattara kada su kasance ƙasa da gram 1-2. Ga marasa lafiya da zawo, idan najasa ruwa ne, da fatan za a tattara aƙalla 1-2 ml na ruwan najasa. Idan najasa ta ƙunshi jini da yawa, da fatan za a sake tattara samfurin.

4.An bada shawarar gwada samfurori nan da nan bayan tattarawa, in ba haka ba ya kamata a aika su zuwa dakin gwaje-gwaje a cikin sa'o'i 6 kuma a adana su a 2-8 ° C. Idan ba a gwada samfuran a cikin sa'o'i 72 ba, ya kamata a adana su a zazzabi da ke ƙasa -15 ° C.

5.Yi amfani da najasa sabo don gwaji, da samfurin najasar da aka gauraye da ruwa mai tsafta ko distilled.